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Deproteinized calf blood extract and preparation process thereof

A technology of protein removal and extraction, which is applied in the field of medicine, can solve the problems of decreased pharmacodynamic activity, high production cost, denaturation and inactivation, etc., and achieve the effects of shortening the production cycle, improving quality standards, and reducing pollution

Inactive Publication Date: 2014-01-08
BEIHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction using the above method not only causes environmental pollution and high production costs, but more importantly, the organic solvent causes the denaturation and inactivation of biologically active compounds, which reduces the pharmacodynamic activity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Under sterile conditions, take 100kg of venous blood from a 5-month-old calf, stir, heat and sterilize, and after squeezing, filter the supernatant through an inorganic membrane (ceramic membrane 7μ); add hydrochloric acid to the 50kg filtrate to adjust the pH value to 3~4 It is acidic, keep stirring, after ultrafiltration, collect the filtrate, add sodium hydroxide to the collected filtrate to adjust it to alkaline 8~10, then ultrafilter (20000 Daltons), collect the filtrate and adjust it to neutral; reverse osmosis Membrane concentrated 25kg; 5000 molecular weight ultrafiltration to remove protein, then sterilized and filled. The detection activity index SI was 4.5.

Embodiment 2

[0015] Under sterile conditions, take 100kg of venous blood from a 6-month-old calf, stir, heat and sterilize, and after squeezing, the supernatant is filtered through an inorganic membrane (ceramic membrane 7μ); add hydrochloric acid to the 55kg filtrate to adjust the pH value to 4 to be acidic , stirring continuously, after ultrafiltration, collect the filtrate, add sodium hydroxide to the collected filtrate to adjust to alkaline 10, then ultrafilter (20000 Daltons), collect the filtrate and adjust to neutral; reverse osmosis membrane concentration 28kg; 5000 molecular weight ultrafiltration to remove protein, then sterilized and filled. The detection activity index SI was 4.3.

Embodiment 3

[0017] Under sterile conditions, take 100kg of venous blood from a 2-month-old calf, stir, heat and sterilize, and after squeezing, the supernatant is filtered through an inorganic membrane (ceramic membrane 7μ); add hydrochloric acid to the 48kg filtrate to adjust the pH value to 3 to be acidic , stirring continuously, after ultrafiltration, collect the filtrate, add sodium hydroxide to the collected filtrate to adjust to alkaline 9, then ultrafilter (20000 Daltons), collect the filtrate and adjust to neutral; reverse osmosis membrane concentration 22kg; 5000 molecular weight ultrafiltration to remove protein, then sterilized and filled. The detection activity index SI was 4.8.

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PUM

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Abstract

The invention provides a deproteinized calf blood extract and a preparation process thereof. The preparation process comprises the following steps of: collecting venous blood of calves which are 1-6 months old; coagulating blood with a physical method; removing proteins from blood by adopting a squeezing method; performing acid and alkali treatment on an obtained liquid; removing acidic and alkaline protein impurities; performing ultrafiltration and sterilization on obtained blood serum; and filling. Due to the adoption of the preparation process, a deproteinized calf blood extract production process is perfected, the breathing vitality is increased by 80 percent, the medicament quality standard is raised, cost is lowered, pollution is lowered, the production period is shortened, the quality standard of a product is raised, and the industrial production goal is achieved.

Description

technical field [0001] The invention relates to a protein-free extract of calf blood and a preparation process thereof, and improves the production process of the protein-free extract of calf blood, belonging to the technical field of medicine. Background technique [0002] Calf blood protein-free extract (protein-free calf blood extract) is the main raw material of calf blood protein-free extract injection. Calf blood deproteinized extract is a small molecule biologically active substance obtained from 1-6 month old calf blood after deproteinization, and its main components are amino acids, small peptides, nucleic acids and other nitrogenous compounds. [0003] Calf blood deproteinized extract can significantly improve the activity of reticuloendothelial system, increase enzyme activity, accelerate cell oxidative phosphorylation reaction and ATP synthesis, activate cell respiration, promote metabolism, and accelerate tissue regeneration. The drug has a positive therapeutic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/14A61K9/08A61P25/00A61P9/10A61P27/02A61K35/16
Inventor 安丽萍杜培革李红宇苑广信韩笑姜爽迟仁志徐广宇邵晓彤周亭亭
Owner BEIHUA UNIV
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