Growth and cultivation matrix used for ecological remediation and preparation method thereof
A cultivation substrate and ecological restoration technology, applied in the application, fertilizer mixture, fertilization device and other directions, can solve the problems of unsustainability and low maintenance, and achieve the effect of reducing maintenance conditions and costs, improving quality and reducing maintenance costs.
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Embodiment 1
[0026] Matrix preparation:
[0027] (1) Preparation of urea-formaldehyde foam particles. The urea-formaldehyde foam (commercially available product) is crushed into uniform particles with a diameter of about 5 mm.
[0028] (2) Preparation of probiotic powder. Bacillus cereus was continuously cultured in a fermenter at 37°C for 16 hours until the O.D600 value of the bacteria was greater than 1.5. Then use a low-temperature high-speed centrifuge to centrifuge at 4000rpm for 1 hour at 2°C to separate the bacteria from the medium, and make the bacillus that has been centrifuged to make a sludge, and finally use a continuous low-temperature drying equipment to make the sludge into an active plant probiotic bacteria powder.
[0029] The composition of the Bacillus cereus culture medium is, by mass percentage, glucose 1.0%, ammonium sulfate 0.2%, dipotassium hydrogen phosphate 0.3%, magnesium sulfate 0.02%, calcium chloride 0.01%, and the balance is water; pH7.2 .
[0030] (3) The...
Embodiment 2
[0036] (1) Preparation of urea-formaldehyde foam particles. The urea-formaldehyde foam (commercially available product) was crushed into uniform particles with a diameter of about 8 mm.
[0037] (2) Preparation of probiotic powder. Bacillus megaterium was continuously cultured in LB medium in a fermenter at 37°C for 16 hours until the O.D600 value of the bacteria was greater than 1.5. Then use a low-temperature high-speed centrifuge to centrifuge at 4000rpm for 1 hour at 2°C to separate the bacteria from the culture medium, and make the bacteria sludge from the Bacillus megaterium precipitated by centrifugation, and finally make the bacteria sludge into active plants through continuous low-temperature drying equipment Probiotic powder.
[0038] (3) The binder is composed of 7g of high molecular weight potassium polyacrylate, 0.5kg of agar powder (both commercially available products) and 1kg of 425# Portland cement.
[0039] (4) The water retaining agent is a mixture of 6g of...
Embodiment 3
[0044] (1) Preparation of urea-formaldehyde foam particles. The urea-formaldehyde foam (commercially available product) was crushed into uniform particles with a diameter of about 3 mm.
[0045] (2) Preparation of probiotic powder: Bacillus cereus and Bacillus megaterium were fermented and cultured in their respective culture media, and cultured continuously at 37°C for 16 hours until the O.D600 value of the bacteria was greater than 1.5. Finally, use a low-temperature high-speed centrifuge at 4000rpm at 2°C for 1 hour to separate the bacteria from the culture medium, and make the bacteria sludge from the centrifuged Bacillus cereus and Bacillus megaterium, and finally separate the bacteria by continuous low-temperature drying equipment. The mud is pulverized, and the pulverized Bacillus cereus and Bacillus megaterium powder are mixed in a mass ratio of 2:1, and set aside.
[0046] (3) The adhesive is composed of 9g high molecular weight potassium polyacrylate, 0.5kg agar powd...
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