Method for measuring artemisinin content in traditional Chinese medicinal materials such as Artemisia annua and samples containing artemisinin components
A technology of artemisinin and determination method, which is applied in the direction of chemical reaction of materials and material analysis by observing the influence on chemical indicators, etc., to achieve the effects of fast measurement, low price, and simple and fast operation.
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Embodiment 1
[0018] Example 1: A method for determining the content of artemisinin in Chinese herbal medicines such as Artemisia annua and samples containing artemisinin components. The method is to dissolve 70.3 mg of standard artemisinin in 5.0 mL of ethanol. Add 0.5g NaHCO to a 50 mL Erlenmeyer flask 3 , 1.0 mL of glacial acetic acid, then 2.0 mL of water, 0.5 g of KI and 2.0 mL of concentrated hydrochloric acid were added to the ethanol solution of the above artemisinin sample, reacted in a water bath at 70°C for 15 min, then cooled to room temperature, and dissolved in 0.1 mol / L Na 2 S 2 o 3 Titrate the standard solution until light yellow, add starch solution as indicator, take Na 2 S 2 o 3 Titration to colorless is the titration end point, and the total consumption of Na 2 S 2 o 3 Standard solution 5.0 mL. The content of artemisinin in the sample was calculated by the formula, and the calculated value of artemisinin content was 70.5 mg.
Embodiment 2
[0019] Example 2: A method for determining the content of artemisinin in Chinese herbal medicines such as Artemisia annua and samples containing artemisinin components. The method is to dissolve 70.9 mg of standard artemisinin in 5.0 mL of ethanol. Add 0.5g NaHCO to a 50 mL Erlenmeyer flask 3 , 1.0 mL of glacial acetic acid, 2.0 mL of water, 0.5 g of KI and 2.0 mL of concentrated hydrochloric acid were added to the ethanol solution of the above artemisinin sample, reacted in a water bath at 70°C for 30 min, then cooled to room temperature, and dissolved in 0.1 mol / L Na 2 S 2 o 3 Titrate the standard solution until light yellow, add starch solution as indicator, take Na 2 S 2 o 3 Titration to colorless is the titration end point, and the total consumption of Na 2 S 2 o 3 The standard solution was 4.9 mL, and the content of artemisinin in the sample was calculated by the formula, and the calculated value of artemisinin content was 69.1 mg.
Embodiment 3
[0020] Example 3: A method for determining the content of artemisinin in Chinese medicinal materials such as Artemisia annua and samples containing artemisinin components, the method is to take artemisinin standard: 69.8 mg dissolved in 5.0 mL ethanol. Add 0.5g NaHCO to a 50 mL Erlenmeyer flask 3 , 1.0 mL of glacial acetic acid, 2.0 mL of water, 0.5 g of KI and 2.0 mL of concentrated hydrochloric acid were added to the ethanol solution of the above artemisinin sample, reacted in a water bath at 70°C for 10 min, then cooled to room temperature, and dissolved in 0.1 mol / L Na 2 S 2 o 3 Titrate the standard solution until light yellow, add starch solution as indicator, take Na 2 S 2 o 3 Titration to colorless is the titration end point, and the total consumption of Na 2 S 2 o 3 The standard solution was 4.8 mL, and the content of artemisinin in the sample was calculated by the formula, and the calculated value of artemisinin content was 67.7 mg.
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