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Method for researching microbial degradation of petroleum pollutants under low-temperature high-salt conditions

A technology of pollutants and petroleum, applied in the direction of biochemical equipment and methods, microorganism measurement/inspection, etc., can solve the problems of unstable microbial film structure and affecting microorganisms

Inactive Publication Date: 2013-01-23
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The increase of salinity will cause the instability of microbial membrane structure and affect the degradation ability of microorganisms to petroleum pollutants

Method used

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  • Method for researching microbial degradation of petroleum pollutants under low-temperature high-salt conditions
  • Method for researching microbial degradation of petroleum pollutants under low-temperature high-salt conditions

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Configure inorganic salt medium, sodium nitrate 0.5g / L, potassium dihydrogen phosphate 0.5g / L, potassium dihydrogen phosphate 0.5g / L, ammonium sulfate 4g / L, NaCl20g / L, magnesium sulfate 2g / L. Add trace elements 5mL / L to adjust the pH to 6.5. Divide 100mL of inorganic salt medium into 250mL Erlenmeyer flasks, seal and sterilize at 121°C for 20 minutes, cool to room temperature, add initial OD 600 0.1 for mycobacterial cp1. Add petroleum hydrocarbons that have been sterilized and passed through a 0.22 micron oil film in the inoculated inorganic salt medium. Placed in a shaker at 5°C and 180rpm for 10 days, the remaining petroleum hydrocarbons were extracted with n-hexane, and the degradation rate of petroleum hydrocarbons was 56.37% by comparing the changes in petroleum hydrocarbon content before and after.

Embodiment 2

[0026] Prepare inorganic salt medium, sodium nitrate 1g / L, potassium dihydrogen phosphate 1g / L, potassium dihydrogen phosphate 1g / L, ammonium sulfate 2g / L, NaCl 30g / L, magnesium sulfate 0.5g / L. Add 10mL / L of trace elements to adjust the pH to 6.5. Divide 100mL of inorganic salt medium into 250mL Erlenmeyer flasks, seal and sterilize at 121°C for 20 minutes, cool to room temperature, add initial OD 6000.1 for mycobacterial cp1. Add petroleum hydrocarbons that have been sterilized and passed through a 0.22 micron oil film in the inoculated inorganic salt medium. Placed in a shaker at 10°C and 200rpm for 12 days, the remaining petroleum hydrocarbons were extracted with n-hexane, and the degradation rate of petroleum hydrocarbons was 87.90% by comparing the changes in petroleum hydrocarbon content before and after.

Embodiment 3

[0028] Prepare inorganic salt medium, sodium nitrate 2g / L, potassium dihydrogen phosphate 1.5g / L, potassium dihydrogen phosphate 1.5g / L, ammonium sulfate 3g / L, NaCl 50g / L, magnesium sulfate 1.5g / L. Add 15mL / L of trace elements to adjust the pH to 7.5. Divide 100mL of inorganic salt medium into 250mL Erlenmeyer flasks, seal and sterilize at 121°C for 20 minutes, cool to room temperature, add initial OD 600 0.1 for mycobacterial cp1. Add petroleum hydrocarbons that have been sterilized and passed through a 0.22 micron oil film in the inoculated inorganic salt medium. Placed in a shaker at 15°C and 250rpm for 16 days, the remaining petroleum hydrocarbons were extracted with n-hexane, and the degradation rate of petroleum hydrocarbons was 63.49% by comparing the changes in petroleum hydrocarbon content before and after.

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Abstract

The invention relates to a method for researching microbial degradation of petroleum pollutants under low-temperature high-salt conditions, which comprises the following steps: preparing a basic inorganic salt culture medium for degrading petroleum pollutants, filling 100mL of inorganic salt culture medium into a 250mL triangular flask, sealing, sterilizing at the high temperature of 121 DEG C for 20 minutes, and cooling to room temperature for later use; adding a low-temperature-resistant high-salt-tolerant strain into the inorganic salt culture medium, wherein the initial OD600 is 0.1; passing petroleum hydrocarbons through a 0.22-micrometer oil film to remove internal infectious microbes, and adding 0.5mL of the petroleum hydrocarbons into the inoculated inorganic salt culture medium; and after carrying out shake culture, taking samples every other 24 hours, and measuring the thallus concentration and remaining petroleum hydrocarbon concentration in the culture medium. The cell biomass variation of mycobacteria cp1 degrading petroleum hydrocarbons under the conditions of 10 DEG C, pH 7.2 and 200rpm proves that the OD600 reaches the maximum value 1.6 at the time of the 192nd hour, and remains constant until the 240th hour. The degradation rate for the petroleum hydrocarbons increases as the time changes, and reaches the maximum value 94.36% at the 240th hour.

Description

technical field [0001] The invention relates to the technical field of petroleum pollution, in particular to a method for studying microbial degradation of petroleum pollutants under low-temperature and high-salt conditions. Background technique [0002] Petroleum is the blood of industry, and as an important energy source and chemical raw material, it plays an irreplaceable role. With the continuous expansion of the scope of petroleum applications, the consumption is increasing day by day. In the process of oil extraction, refining, storage and transportation, due to the limitation of technology and technical level and the impact of unexpected accidents, a large amount of oil and oily waste residue and wastewater are entering the environment, which seriously affects the ecological environment. balance. With the further expansion of the scale of ocean transportation of oil and the development of offshore oil extraction industry, the ocean has become the hardest hit area of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/64C12Q1/02
Inventor 贾晓强周征西闻建平
Owner TIANJIN UNIV
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