Test card for rapidly diagnosing pancreatic trauma and acute pancreatitis and preparation method thereof

Abstract

The invention discloses a test card for rapidly diagnosing pancreatic trauma and acute pancreatitis and a preparation method of the test card. The test card comprises a plastic shell, a bottom lining and detection modules, wherein the plastic shell is provided with a sampling hole and an observation window, and the sampling hole and the observation window are fixed with transparent waterproof films. The bottom lining is provided with multiple longitudinally parallel arranged slots; and the number of the detection modules is consistent to that of the bottom lining, and the detection modules are glued into the slots of the bottom lining. Each detection module comprises chromatographical membranes, combination pads, sample pads, filtration pads and water sucking pads. The chromatographical membranes are provided with test lines and quality control lines, the test lines are TAP (thiamphenicol) polyclonal antibodies, and the quality control lines are covered rabbit anti-mice immune globulin antibodies. The test card for rapidly diagnosing pancreatic trauma provided by the invention is capable of quickly and accurately diagnosing the diseases such as pancreatic trauma and acute pancreatitis, and adding multiple samples simultaneously for positive control and negative control, and has the advantages of being convenient and rapid to detect, high in flexibility and accuracy, stable in property and cheap in cost.

Description

technical field [0001] The invention relates to the field of clinical diagnosis, in particular to a test card for rapidly diagnosing pancreatic trauma and acute pancreatitis and a preparation method thereof Background technique [0002] Pancreatic trauma and acute pancreatitis are common clinical emergencies and critical illnesses. If they are not treated in time, they often endanger the lives of patients. Therefore, timely diagnosis of pancreatic trauma and acute pancreatitis is very important to save patients' lives. [0003] At present, the routine diagnosis of pancreatic trauma and acute pancreatitis is mainly to measure amylase activity in peritoneal exudate and blood, and the sharp increase in its activity is used as the basis for diagnosis, but the accuracy of this method is not good, because there are still some patients There was no major change in the measured amylase activity. In addition, pancreatic trauma and acute pancreatitis are diagnosed by the APACHE II sc...

AI Technical Summary

Problems solved by technology

[0005] The current detection method for TAP is mainly enzyme-linked immunosorbent assay (ELISA), but this method takes a long time (4 to 5 hours), requires specific absorbance detection instruments and professional personnel to operate, and is also expensive, so It cannot meet the requirements for rapid diagnosis of the disease, and it is not suitable for use in primary medical institutions.

The applicant found in use that the invention patent application with publication number CN101609093A has the following problems in actual use. Because the chromatographic membrane, water-absorbing pad, and filter pad are all made of materials that are easy to absorb moisture, they are often used in actual use due to the high environmental humidity and Longer storage time reduces the performance of the material, which in turn affects the test accuracy of the test card. The experiment found that about 100 samples were tested using the test card according to CN101609093A, and nearly 15% of false positives ( error rate), and using the test card according to CN101609093A can not do parallel and contrast tests on the samples to be tested simultaneously, which reduces the accuracy of the test

It is even more impossible to achieve a roughly quantitative effect on the test sample

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