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Method for extracting small-molecule peptides of spirulina

A technology of small molecule peptides and spirulina, which is applied in the fields of peptides, food preparation, food science, etc., can solve the problems of complex equipment requirements, long time for small molecule peptides, high technical requirements, etc., to shorten the time required for extraction and simplify extraction. The procedure of extraction, the effect of remarkable economic benefit

Inactive Publication Date: 2013-01-16
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These tasks have high technical requirements, complex equipment requirements, and a long time. Even skilled technicians need a few days or weeks, and less familiar technicians need longer time to complete the extraction of small molecule peptides.
More importantly, the polypeptides of living cells have partially lost their original activity after such a long process of extraction, separation and purification, resulting in half the result with twice the effort.

Method used

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  • Method for extracting small-molecule peptides of spirulina
  • Method for extracting small-molecule peptides of spirulina
  • Method for extracting small-molecule peptides of spirulina

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Example 1 The method for extracting small molecule peptides from Spirulina (1)

[0031] (1) Weigh spirulina powder (purchased from Inner Mongolia Zaihuishou Biological Engineering Co., Ltd.) with a protein content of 65%, dissolve it in distilled water, and prepare a solution with a material-to-liquid ratio of 2g / L, pH=7, and then use a magnetic stirrer 600r / min the solution was stirred for 40 min and centrifuged at 4000 r / min for 30 min.

[0032] (2) Take 400ml of the supernatant and put it into the foam separation column for foam separation, the air flow rate is 3.5L / min, collect the foam part and wait for defoaming to obtain a solution mainly containing small molecular peptides of spirulina, and the protein dissolution rate is 3.0% , its enrichment ratio is 1.6, and its recovery rate is 80%.

Embodiment 2

[0033] Example 2 The method for extracting small molecule peptides from Spirulina (2)

[0034] (1) Weigh spirulina powder (purchased from Inner Mongolia Zaihuishou Biological Engineering Co., Ltd., protein content 65%) and dissolve in distilled water to prepare a solution with a material-to-liquid ratio of 0.5 g / L. Control the pH=9.5, then stir the solution with a magnetic stirrer at 600r / min for 40min, and centrifuge at 8000r / min for 15min.

[0035] (2) Take 400ml of the supernatant and put it into the foam separation column for foam separation. The air flow rate is 1.5L / min. After the foam part is collected and defoamed, it will mainly contain Spirulina small molecule polypeptide, and the protein dissolution rate is 15.0%. Its enrichment ratio is 1.8, and its recovery rate is 90%.

Embodiment 3

[0036] Example 3 The method for extracting small molecule peptides from Spirulina (3)

[0037] (1) Weigh spirulina powder (purchased from Inner Mongolia Zaihuishou Biological Engineering Co., Ltd., protein content 65%) and dissolve it in distilled water to prepare a solution with a material-to-liquid ratio of 8g / L. Control the pH=5.1, then stir the solution with a magnetic stirrer at 600r / min for 40min, and centrifuge at 5000r / min for 20min.

[0038] (2) Take 400ml of the supernatant and put it into the foam separation column for foam separation. The air flow rate is 2L / min. After the foam part is collected and defoamed, it will mainly contain Spirulina small molecule polypeptide. The protein dissolution rate is 6.5%. The enrichment ratio is 4.3, and the recovery rate is 20%.

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Abstract

The invention provides a method for extracting small-molecule peptides of spirulina. The method includes: preprocessing the spirulina, adjusting the feed-liquid ratio to be 0.5-8g / L and the pH (potential of hydrogen) value to be 5-10, and performing foam separation of the spirulina after sufficient mixing so as to obtain foam extracting solution, namely small-molecule bioactive peptides with the molecular weight of 5000Da. The dissolution rate of spirulina protein obtained by the method can reach 12.6%, enrichment ratio can reach 4.4, and the highest recovery rate can reach 98%. Compared with a traditional method, the method for extracting the small-molecule active peptides is quite simple in process and does not need expensive equipment and apparatuses, extracting procedures are greatly simplified, extracting cost is greatly saved, and economic benefits are remarkable.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for extracting small molecule peptides from spirulina. Background technique [0002] Spirulina is a low-level phytoplankton prokaryotic plant that has existed on the earth for more than 3 billion years. Its appearance is blue-green and spiral-shaped under a microscope, so it gets its name. Spirulina is an alkaline nutritious food, which can change the acidic body of the human body into a healthy weakly alkaline body. More importantly, spirulina is rich in various nutrients necessary for the human body, and the composition is relatively balanced. Among them, the protein content is the highest among all natural plants known so far, accounting for about 60% to 70% of the dry weight. On the banks of Lake Chad in Africa and Lake Texcoco in Mexico, local people have used spirulina as a food source for hundreds of years. As an excellent nutritional supplement for humans and anima...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K2/00A23L1/305
Inventor 李博生郭准
Owner BEIJING FORESTRY UNIVERSITY
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