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Detection method of authentic rheum officinale seeds

A detection method and seed technology, which is applied in the detection field of authentic rhubarb seeds, can solve problems such as difficult to replace detection methods, difficult accurate measurement, and insufficient accuracy, and achieve good application prospects, simple detection method, and high accuracy.

Active Publication Date: 2013-01-02
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology helps identify if there're really any roots or seed that might have been found containing true rootlets by measuring their size beforehand. It also checks how well these seals were mixing up when they had gone through various stages during production. By comparing this data against known samples, we could determine who made those seeds actually wrongly. Overall, this technology provides an accurate way to check where fake ones may come into contact with them while avoiding being mistaken about what was happening earlier on top.

Problems solved by technology

The technical problem addressed in this patents relates to finding an effective way to distinguish between genuinary (fake), fake ones, and mixed rhubsauriculus seeds without requiring expensive equipment like X ray fluorescence scanning machines.

Method used

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  • Detection method of authentic rheum officinale seeds
  • Detection method of authentic rheum officinale seeds
  • Detection method of authentic rheum officinale seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 Detection method of the present invention

[0031] 1. Detection method

[0032] (1) Take the seeds to be inspected;

[0033] (2) Take 0.15g of seed powder to be tested (passed through No. 4 sieve), accurately weigh it, put it in a stoppered Erlenmeyer flask, accurately add 22.5ml of methanol, weigh it, heat and reflux for 30min, let it cool, and weigh it again , make up the lost weight with methanol, shake well, and filter. Precisely measure 5ml of continued filtrate, put it in a flask, evaporate the solvent, add 5ml of 6% hydrochloric acid solution, ultrasonicate for 1 minute, add 5ml of chloroform, heat to reflux for 30min, let it cool, put it in a separatory funnel, and use a small amount of three Wash the container with methyl chloride, put it into a separatory funnel, separate the chloroform layer, extract the acid solution with chloroform for 3 times, 5ml each time, combine the chloroform solution, recover the solvent under reduced pressure until dr...

Embodiment 2

[0037] Embodiment 2 detection method of the present invention

[0038] 1. Detection method

[0039] (1) Take the seeds to be inspected;

[0040] (2) Take 0.15g of the seed powder to be tested (passed through a No. 4 sieve), accurately weigh it, put it in a stoppered Erlenmeyer flask, add 25ml of methanol accurately, weigh it, heat it to reflux for 1 hour, let it cool, and weigh it again , make up the lost weight with methanol, shake well, and filter. Precisely measure 5ml of the continued filtrate, put it in a flask, evaporate the solvent, add 10ml of 8% hydrochloric acid solution (the concentration of hydrogen ions in the 8% hydrochloric acid solution is 2.5mol / L), ultrasonicate for 2 minutes, and then add 10ml of chloroform, Heat to reflux for 1 hour, let it cool, put it in a separatory funnel, wash the container with a small amount of chloroform, put it into the separatory funnel, separate the chloroform layer, and extract the acid solution 3 times with chloroform, 10ml e...

Embodiment 3

[0044] Embodiment 3 detection method of the present invention

[0045] 1. Detection method

[0046] (1) Take the seeds to be inspected;

[0047] (2) Take 0.15g of the seed powder to be tested (passed through the No. 4 sieve), accurately weigh it, put it in a stoppered Erlenmeyer flask, add 27.75ml of methanol accurately, weigh it, heat it to reflux for 90min, let it cool, and weigh it again , make up the lost weight with methanol, shake well, and filter. Accurately measure 5ml of the continued filtrate, put it in a flask, evaporate the solvent, add 15ml of 10% hydrochloric acid solution, ultrasonicate for 3 minutes, add 15ml of chloroform, heat to reflux for 90min, let it cool, put it in a separatory funnel, and use a small amount of three Wash the container with methyl chloride, put it into a separatory funnel, separate the chloroform layer, extract the acid solution with chloroform for 3 times, 15ml each time, combine the chloroform liquid, recover the solvent under reduce...

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Abstract

The invention discloses a detection method of authentic rheum officinale seeds, which adopts a high-efficient liquid-phase chromatography to detect, and the detection method comprises the following steps of (1) taking seeds to be detected, grinding the seeds, extracting the seeds through methanol, filtering to obtain the filter liquid, removing methanol, hydrolyzing with acid, extracting with organic solvent to obtain an organic solvent layer, recycling the solvent until being dried, and re-dissolving to prepare sample solution; (2) taking aloe-emodin reference substances to be dissolved to prepare reference substance solution; (3) utilizing the high-efficient liquid-phase chromatography to detect, wherein the colored peak in chromatogram map of the sample solution is maintained consistent with the colored peak of the aloe-emodin reference substances on the aspect of the time, and determining whether the authentic rheum officinale seeds are contained in the seeds to be detected or not, wherein the chromatography condition is as follows: solid phase: octadecylsilane chemically bonded silica, flow phase: methanol-0.1 percent phosphoric acid solution, the volume ratio of the solid phase and the flow phase is 80 to 90: 10 to 20; and the detection wavelength: 254nm. The detection method of the authentic rheum officinale seeds is high in accuracy, convenient in operation, low in cost and good in market application prospect.

Description

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Claims

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Application Information

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Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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