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Lactobacillus strain lactobacillus parafarraginis ZH1 and application thereof

A technology of lactic acid bacteria and microbial strains, applied in the directions of application, bacteria, microorganisms, etc., can solve the problem of insignificant effect, and achieve the effect of unique effect, easy utilization, and overcoming easy aerobic deterioration.

Active Publication Date: 2013-01-02
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its effect of inhibiting silage under certain conditions is not obvious, such as low temperature and short-term storage

Method used

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  • Lactobacillus strain lactobacillus parafarraginis ZH1 and application thereof
  • Lactobacillus strain lactobacillus parafarraginis ZH1 and application thereof
  • Lactobacillus strain lactobacillus parafarraginis ZH1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 screens the good lactic acid bacteria of high yield benzoic acid and hexadecanoic acid

[0026] The lactic acid bacteria strain ZH1 isolated from Sudan grass silage was subjected to Gram staining and cell shape observation, and was tested according to growth temperature (10, 15, 20, 25, 30, 35, 40, 45, 50°C) and growth pH ( 3.5, 3.75, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0), physiological and biochemical experiments such as benzoic acid and hexadecanoic acid produced in MRS. The results showed that the ZH1 strain was a Gram-positive, heterofermentative bacillus that could grow at 15-45°C and pH4.0-8.5, and had strong acid resistance (Table 1); it could ferment most sugars ( Table 2); in MRS can produce more benzoic acid and hexadecanoic acid ( figure 1 and figure 2 ).

[0027] project characteristic Gram stain detection Positive Catalase activity Negative shape Rod fermented glucose form Shaped ...

Embodiment 2

[0040] The identification of embodiment 2 lactic acid bacteria

[0041] The bacterial strain obtained in Example 1 was cultured overnight at 37°C in 5 mL of MRS medium, and the bacterial solution was transferred into a 1.5 mL centrifuge tube, centrifuged at 10000 rpm / min for 3 min to 5 min to collect the bacteria, and TE 0.1 (10 mmol / L Tris–HCl, 0.1 mmol / L EDTA, pH 8.0) was washed twice, and DNA was extracted with TIANamp Bacteria DNA Kit (TIANGEN BIOTECH CO., LTD, Beijing, China) and detected at OD600 nm its absorbance value. Then, carry out PCR amplification, 16S rDNA amplification primers are 25f (SEQ ID NO:1) and 1492r (SEQ ID NO:2), RCR reaction is 95°C (5 min) - 94°C (30 s) - 55 °C (1 min)-72 °C (1.5 min)-72 °C (10 min), 94 °C (30 s)-55 °C (1 min)-72 °C (1.5 min) reaction cycle 30 times. The amplified product was sent to Huada Gene Co., Ltd. (China) for sequencing, and the results were compared on the NCBI gene bank to find out the standard strain Lactobacillus zeae ...

Embodiment 3

[0042] Example 3 Effects of adding to silage

[0043] Silage addition experiments were carried out with lightly aired and non-aired yellow oats and corn stalks as materials. Cut the material to 1 cm~2 cm, mix evenly, and add about 1.0×10 strain ZH1 per kg of material 8 indivual. Add the material after the bacterial strain ZH1 identified in Example 2 into a polyethylene silage bag of 30 cm × 20 cm, and pack 6 bags for each treatment. 3 bags are stored at 45° C., and 3 bags are stored at 15° C., about 200 g per bag. Air was pumped and sealed with a vacuum sealing machine, and the yellow oat silage was stored at 15°C for 45 days.

[0044] Under the low temperature storage condition of 15℃, both the non-dried yellow oat silage and the sun-dried yellow oat silage produced more lactic acid and ammoniacal nitrogen, less acetic acid, more yeast and less than 40% aerobic stability. Hours; the yellow oat silage added with Lactobacillus Brucella also produced more lactic acid, le...

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Abstract

The invention discloses a lactobacillus strain which is lactobacillus parafarraginis ZH1, and an application thereof. The lactobacillus strain provided by the invention is collected at China General Microbiological Culture Collection Center with an address of Institute of Microbiology, Chinese Academy of Sciences, No.3, Court 1, West Beichen Road, Chaoyang District, Beijing. The strain has a collection number of CGMCC NO.6079. A collection date is May 3rd, 2012. The lactobacillus strain provided by the invention is a Gram stain positive bacilli. The lactobacillus strain is glucose-heterofermentative, and is acid-resisting. The strain has high growth speed and wide reproductive temperature range. The strain has high yield of benzoic acid and palmitic acid. With the strain, silage fermentation aerobic deterioration can be substantially inhibited.

Description

technical field [0001] The invention relates to the field of microbial application and feed preparation and processing, in particular to a strain of lactic acid bacteria Lactobacillus parafarraginis ZH1 and its application in silage preparation. Background technique [0002] Silage is yellow-green in color, sour and fragrant, soft and juicy, and has good palatability. It is widely used in animal husbandry production, especially in ruminant breeding. It has become an indispensable basic feed. However, after the silage container is unsealed, the anaerobic environment immediately becomes an aerobic environment, and aerobic microorganisms begin to proliferate, thereby causing a series of biochemical changes in the silage, which often lead to heating and deterioration of the silage, that is, aerobic deterioration of the silage Phenomenon. Typically, aerobic deterioration is manifested primarily by temperature rise and the appearance of fungal plaques. The rate of aerobic deter...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/14C12R1/225
Inventor 张建国刘秦华
Owner SOUTH CHINA AGRI UNIV
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