Method for extracting isoflavone in soybean germ with aid of extrusion and expansion pretreatments

Abstract

The invention relates to a method for extracting isoflavone in soybean germ with the aid of extrusion and expansion pretreatments. The method comprises the following steps of: adjusting the material water content of soybean germ powder to 5-10%, and performing extrusion and expansion on the soybean germ by using a double-screw extruder, wherein the double-screw extruder has the sleeve temperature of 25-45 DEG C, the screw rotation speed of 60-120rpm and the die orifice aperture of 5-25mm; mixing an expansion product with water, adding protease to carry out enzymolysis, and performing centrifugal separation after the enzymolysis to obtain soybean oil, hydrolysate and dregs, wherein the enzymolysis is carried out under the conditions as follows: enzymolysis pH value: 8-10, enzymolysis temperature: 20-60 DEG C, enzyme adding amount: (1:3)-(1:7), material solution ratio: (1:3)-(1:7), and enzymolysis time:3-7h; adding absolute ethyl alcohol in the hydrolysate and the dregs, extracting isoflavone by ultrasonic waves, wherein the volume ratio of the absolute ethyl alcohol to the hydrolysate is (1-5):1; purifying isoflavone by using macroreticular resin, and eluting to obtain eluate; and rotatably evaporating the eluate, and freezing to be dry to obtain isoflavone. The method provided by the invention has the following characteristics of simple steps, high quality of isoflavone products and high extracting rate.

Description

technical field [0001] The invention relates to a method for extracting soybean isoflavones, in particular to a method for assisting the extraction of soybean isoflavones in soybean germ by extrusion pretreatment, and belongs to the field of separation and purification of natural chemical substances. Background technique [0002] Soybean isoflavones are secondary metabolites formed during the growth of soybeans. They are bioflavonoids, mainly referring to a mixture of polyphenolic compounds with 3-phenylpropanone as the core, which cannot be synthesized in the human body. The distribution in nature is limited, and it mainly exists in leguminous plants, and soybean has the highest content, so it is called soybean isoflavones. Studies have shown that soy isoflavones have a variety of physiological functions and medicinal value. Pharmacological studies have shown that soy isoflavones have estrogen-like effects, which can be used to improve and treat menopausal syndrome in women...

AI Technical Summary

Problems solved by technology

However, the traditional organic solvent extraction method or extraction under heating conditions has the disadvantages of time-consuming operation, large amount of solvent, and high cost.

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