Amplification primer of tridacnidae shellfish mitochondria COI (cytochrome oxidase I) gene
A gene amplification and mitochondrial technology, applied in the direction of DNA / RNA fragments, recombinant DNA technology, etc., can solve the problems of low amplification efficiency, large variation, primers hinder molecular identification research, etc., and achieve the effect of efficient amplification
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[0007] The screening method of the Tridacnaceae shellfish mitochondrial COI gene amplification primer of the present invention adopts four steps: a, on the National Center for Biotechnology Information of the United States (NCBI, http: / / www.ncbi.nlm.nih.gov / ) Download the existing Tridacnaceae sequence; b, use the COI universal amplification primers (LCO1490F and HCO2198R, Folmer 1994) to amplify the COI sequences of some Tridacnaceae species; c, from the Tridacnaceae mtCOI sequence obtained above, Find the conserved sequence fragments by comparison, and synthesize multiple sets of amplification primer sequences; d, screen out a pair of primers with the best effect of amplifying the Tridacaceae mitochondrial COI gene from the multiple sets of synthetic amplification primer sequences.
[0008] The present invention will be further described below in conjunction with embodiment:
[0009] 1. Download 52 COI sequences of 5 species of Tridacnaceae molluscs from the National Center ...
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