Muricidae mitochondria 16s rRNA gene amplification primer
An amplification primer, mitochondrial technology, applied in DNA preparation, recombinant DNA technology and other directions, can solve the problems of large inter-species variability, low amplification efficiency of 16SrRNA non-specific amplification primers, and hindering the use of molecular technology.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0007] The screening method of the amplification primer of the snail family mitochondrial 16S rRNA gene of the present invention adopts three steps: a, utilize 16S rRNA universal amplification primer to amplify the 16S rRNA sequence of the part snail family species; b, obtain from above In some 16S rRNA sequences of Mururaceae, find out the conserved sequence fragments by comparison, and synthesize multiple sets of amplification primer sequences; c, screen and amplify the mitochondrial 16S rRNA gene of Mururaceae from the multiple sets of synthetic amplification primer sequences The best pair of primers.
[0008] The present invention will be further described below in conjunction with embodiment:
[0009] 1. Sample collection: From 2005 to 2010, a total of 160 individuals of 10 genera and 25 species of Murexaceae were collected from the north and south coasts of China.
[0010] 2. DNA extraction: DNA extraction was performed on the collected samples by CTAB method.
[0011]...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com