Rapid identification method for African swine fever virus gene type II and non-type II and application of rapid identification method
A technology of African swine fever virus and genotyping, which is applied in the field of rapid identification of African swine fever virus genotype II and non-II, can solve the problems of high lethality and impact, and achieve low cost, high amplification efficiency, and detection fast effect
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[0040] Embodiment African swine fever virus genotype II type and the characteristic difference sequence of non-II type
[0041] Through the ClustalW multiple sequence alignment of 24 genotypes of African swine fever virus and the B646L gene sequence of the popular strain in my country, the comparison results are shown in figure 1 , figure 1 It shows that ASFV genotype II and non-II type have a characteristic sequence of "C / T" at the 1500 position of B646L gene, wherein ASFV genotype II is C, and non-II type is T.
[0042] The rapid identification method of embodiment African swine fever virus genotype II type and non-II type
[0043] Synthesize the partial sequence of the African swine fever virus gene type II B646L gene and the partial sequence of the African swine fever virus gene type II B646L gene respectively, connect the sequences into the vector (such as pUC57) respectively, extract the plasmid, measure the concentration and dilute to the same concentration as a PCR t...
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