Novel protein and gene that codes therefor

Abstract

Provided is a novel protein that exhibits neuraminidase activity and / or [beta]-galactoside-[alpha]2,6-sialyltransferase activity. Also provided are a nucleic acid that codes for said protein, a vector containing a nucleic acid that codes for said protein, a host cell transformed by said vector, a method for producing recombinant [beta]-galactoside-[alpha]2,6-sialyltransferase, and an antibody that specifically recognizes the aforementioned protein.

Description

technical field [0001] The present invention relates to a protein having neuraminidase activity and / or β-galactoside-α2,6-sialyltransferase activity, a nucleic acid encoding the protein, and a method for producing the enzyme using a microorganism transformed with a gene encoding the protein Method, and antibody specifically recognizing the protein. Background technique [0002] Sugar chains are compounds composed of various sugars, such as monosaccharides such as galactose and N-acetylglucosamine. Sugar chains such as glycoproteins and glycolipids (hereinafter also referred to as complex sugar sugar chains) have very important functions in living bodies. Based on previous studies, it is considered that, among sugar chains, particularly, sugar chains containing monosaccharides called sialic acid exhibit important functions. For example, it has been shown mainly in mammalian cells that sugar chains containing sialic acid are important molecules that function as differentiati...

AI Technical Summary

Problems solved by technology

[0006] However, the most known sialyltransferases derived from marine microorganisms show that their optimum reaction temperature is below 30°C, and the enzyme activity will be rapidly lost in the temperature range above this

Generally, the optimum temperature for the growth of mammalian cells is around 37°C. Therefore, it is necessary to transform animal cells with an expression vector incorporating sialyltransferase from marine bacteria, and allow the animal cells to produce and breed in a suitable environment. However, there is a problem that even if the sialyltransferase protein derived from marine microorganisms is expressed in these cells, the enzymatic activity cannot be obtained

In addition, the synthesis of sugar chains is important in elucidating the functions of new sialic acid-containing sugar chains, however, there are still many problems in the synthesis of sugar chains

As one of the means to solve this problem, it has been considered to combine various animal-derived glycotransferases and sugar acceptors with a wide range of substrate specificity and optimum temperature for which a large number of genes have been obtained and expressed as recombinant enzymes. It is a sialyltransferase derived from a microorganism around 37°C, but there is no report on a sialyltransferase derived from a marine microorganism with an optimum temperature around 37°C

Images