Method for conversing steviolbioside to rebaudiodside B

A technology of stevioside and Aspergillus niger enzyme, which is applied in the field of food chemical industry to achieve the effect of low cost and easy scale-up

Inactive Publication Date: 2012-11-28
成都南诺格生物科技有限责任公司
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  • Summary
  • Abstract
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  • Claims
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Problems solved by technology

[0009] So far, there is no report on the production of rebaudioside B by biotransformation with sucrose as a glycosyl donor using stevioside as a raw material through microbial fermentation

Method used

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  • Method for conversing steviolbioside to rebaudiodside B
  • Method for conversing steviolbioside to rebaudiodside B
  • Method for conversing steviolbioside to rebaudiodside B

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Embodiment Construction

[0029] In a specific embodiment, steviolbioside is abbreviated as SB, rebaudioside B is abbreviated as RB, and sucrose is abbreviated as SU.

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Abstract

The invention discloses a method for conversing steviolbioside to rebaudiodside B, which belongs to the food chemical field, steviolbioside is taken as a raw material, cane sugar is taken as a carbohydrate source, so that the rebaudiodside B can be conversed under the catalysis of aspergillns niger III. According to the invention, the conversion rate of the rebaudiodside B which is obtained by taking steviolbioside as the raw material is greater than or equal to 70%, the rebaudiodside B content in a solid content can reach at least 60%, and can reach 80% after further separation and purification, and even can reach greater than 90%, the rebaudiodside B tastes odorless, and can be individually used as a sweetener, the rebaudiodside B produced by the method has the advantages of low cost and easy amplification, and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of food chemical industry, and in particular relates to a method for converting stevioside into rebaudioside B. Background technique [0002] Steviol glycosides (Steviol glycosides) is a general term for stevia extracts. Its sweet ingredients include stevioside (Stevioside), rebaudioside A, B, C, D, E (Rebaudioside A / B / C / D / E), steviol monoside (Steviolmonoside), steviolbioside (Steviolbioside), dulcoside A (Dulcoside A), etc., the structural formula of steviol glycoside components is as follows: [0003] [0004] Rebaudioside B (Rebaudioside B, hereinafter referred to as RB) is one of the sweet components in steviol glycosides. Studies have found that in addition to strong sweetness, it also has a physiological effect of lowering blood lipids. It is a new generation of functional sweetness agent. However, the content of RB in steviol glycosides is less than 1%. If RB is produced by purifying steviol glycosides, ...

Claims

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Application Information

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IPC IPC(8): C12P19/56C12R1/685
Inventor 李沁桐欧阳劲
Owner 成都南诺格生物科技有限责任公司
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