Application of ethanol in promoting plant growth
A technology for promoting plant growth and ethanol, applied in the directions of plant growth regulators, plant growth regulators, applications, etc., can solve the problems of inconvenient use, unsuitable storage for a long time, time-consuming and laborious, etc., to improve photosynthetic rate, promote plant growth, Easy-to-use effects
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Embodiment 1
[0028] Embodiment 1: the application of the ethanol treatment agent that promotes the growth of rape comprises the steps:
[0029] (1) Use absolute ethanol to prepare an ethanol solution with a concentration of 5% by volume;
[0030] (2) In this experiment, the Brassica napus varieties Yuyou No. 2, Qinyou No. 2, Qianyou No. 2 and A35 were used as test materials; rape seeds were sown in seedling trays, and after two weeks of cultivation, seedlings of uniform size were selected Carry out pot culture, each pot is equipped with humus and perlite (mixing ratio is 1:2);
[0031] (3) After the potted plants continue to be cultivated for one week, spray evenly on the leaves of rapeseed with 5% ethanol solution, and spray 5% (v / v) At the same time, set up a control group (CK)---spray 20ml of distilled water; the time of spraying is twice a week, once every Monday and Thursday at 18 o'clock in the afternoon; after spraying, cover it with plastic cloth overnight , to prevent the volati...
Embodiment 2
[0032] Embodiment 2: the mensuration of the rape photosynthetic parameter of foliar application treatment agent, comprises the steps:
[0033] A. Use the American CID CI-340 handheld photosynthesis measurement system to measure the net photosynthetic rate of the third leaf (the third leaf counted from top to bottom of the plant). Measure 3-5 leaves for each experimental group, whichever is the best average value;
[0034] B, with U.S. CID CI-340 hand-held photosynthesis measurement system, measure the transpiration rate of the 3rd leaf, each experimental group measures 3-5 blade, gets its average value;
[0035] C, with U.S. CID CI-340 hand-held photosynthesis measuring system, measure the stomatal conductance of the 3rd leaf, each experimental group measures 3-5 blades, gets its average value;
[0036] D. Use the American CID CI-340 handheld photosynthesis measurement system to measure the intercellular CO of the third leaf 2 Concentration, each experimental group measur...
Embodiment 3
[0043] Embodiment 3: the mensuration of the rape chlorophyll content of leaf application treatment agent, comprises the steps:
[0044] A. Take the third leaf of different varieties of rapeseed, weigh the weight of the leaf, cut it into several sections, put it into 25ml of acetone and ethanol extract (acetone V:ethanol V=1:1), extract at room temperature and dark for 24 hours ;
[0045] B. Measure the absorbance value of the extract at 645nm and 663nm (A);
[0046] C. Calculate the content of chlorophyll according to the following formula:
[0047] Chlorophyll a content (mg.g -1 ) = (12.7×A663-2.59×A645)×V / (M×1000);
[0048] Chlorophyll b content (mg.g -1 ) = (22.9×A645-4.67×A663)×V / (M×1000);
[0049] Total chlorophyll content (mg.g -1 )=(20.3×A645+8.04×A663)×V / (M×1000).
[0050]Among them, M is the fresh weight of the leaves, and the unit is g; CK is the control group—sprayed with distilled water. (See Figure 5-7 )
[0051] Figure 5 Show, the treatment group o...
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