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Culture medium for insect cell growth

A technology of insect cells and culture medium, which is applied in the field of culture medium and cell culture medium, can solve the problems of large differences in insect cell growth, etc., and achieve the effects of simple preparation method, easy-to-obtain raw materials, and scientific and reasonable formula

Inactive Publication Date: 2012-11-14
THE RES INST OF RESOURCES INSECTS RIRI OF THE CHINESE ACADEMY OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are a variety of commercial media available, the growth of different insect cells in different media is quite different, and the media that can adapt to the growth of a variety of insect cells is still relatively rare, especially in new insect cells. In terms of the establishment of the line, finding a suitable medium is a big difficulty

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Prepare the following materials according to the amount of 1000 ml medium:

[0021] Purified water 800ml

[0022] Commercial Grace medium 40g

[0023] Commercial trehalose 3.0g

[0024] Commercial yeast extract 0.5g

[0025] Commercially available fetal bovine serum 200ml;

[0026] Through the following methods:

[0027] Dissolve 45 grams of commercially available Grace medium dry powder in an appropriate amount of purified water; add 3 g of commercially available trehalose Glucose and 0.5 g of commercially available yeast extract TC-Yeastolate in sequence; adjust the pH value to 6.1 with 1N NaOH, add 200ml of commercially available fetal bovine serum was added to 1000ml of purified water, filtered and sterilized as usual to obtain a medium suitable for the growth of various insect cells for different purposes.

Embodiment 2

[0029] Prepare the following materials according to the amount of 1000 ml medium:

[0030] Purified water 850ml

[0031] Commercial Grace medium 50g

[0032] Commercial trehalose 0.5g

[0033] Commercial Yeast Extract 3.0g

[0034] Commercially available fetal bovine serum 150ml;

[0035] Through the following methods:

[0036] Dissolve 50 g of commercially available Grace medium dry powder in an appropriate amount of purified water; add 0.5 g of commercially available trehalose Glucose and 3 g of commercially available yeast extract TC-Yeastolate in sequence; adjust the pH value to 6.3 with 1N HCl, add 150ml of commercially available fetal bovine serum was added to 1000ml of purified water, filtered and sterilized as usual to obtain a medium suitable for the growth of various insect cells for different purposes.

Embodiment 3

[0038] Prepare the following materials according to the amount of 1000 ml medium:

[0039] Purified water 900ml

[0040] Commercial Grace medium 50g

[0041] Commercial trehalose 2.0g

[0042] Commercial yeast extract 2.0g

[0043] Commercially available fetal bovine serum 100ml;

[0044] Through the following methods:

[0045] Dissolve 50 grams of commercially available Grace medium dry powder in an appropriate amount of purified water; add 2 g of commercially available trehalose Glucose and 2 g of commercially available yeast extract TC-Yeastolate in sequence; adjust the pH value to 6.2 with 1N NaOH, and add 100 ml Commercially available fetal bovine serum, add purified water to make up 1000ml, filter and sterilize according to routine, to obtain a medium suitable for the growth of various insect cells for different purposes.

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Abstract

The invention provides a culture medium for insect cell growth, which is characterized in that the culture medium per 1,000ml comprises the following components: 40-50g of Grace culture medium, 0.5-3g of trehalose, 0.5-3g of yeast extract, 100-200ml of fetal calf serum and the balance of water. The culture medium obtained by using the technical scheme has the advantages of scientific and reasonable formulation, easily obtained raw materials and simplicity in a preparation method; after 14 insect cells are cultured, a result shows that may insect cells from lepidoptera, coleoptera, orthoptera, hymenoptera, diptera, homoptera and the like are suitably cultured by using the culture medium provided by the invention, the majority of cells can grow in the culture medium provided by the invention, and a plurality of finite cell lines and infinite cell lines are successfully established.

Description

[0001] Technical field [0002] The invention involves a medium, especially a medium that is suitable for the growth of multiple insect cells, which belongs to the field of cell culture medium technology. Background technique [0003] As a technology with industrialization development, insect cell culture is increasingly attracting people's attention.Insect cell culture medium is a key substance for cultivating insect cells. The growth, value -added, and differentiation of insect cells require a medium. The suitable medium will play an extremely important role in the growth and proliferation of insect cells.The cultivation effect enables primary culture to maintain a single -layer cell with high density and paste.During the growth of insect cells, multi -faceted nutrients are required, such as a variety of amino acids, vitamins, coenzymes, nucleic acids, purine, pyrimidine, hormones and growth factors.Most insect cells use glucose, fructose or maltose as the main carbon source an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/07C12R1/91
Inventor 张欣冯颖丁伟峰李娴马涛孙龙何钊
Owner THE RES INST OF RESOURCES INSECTS RIRI OF THE CHINESE ACADEMY OF FORESTRY
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