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Construction method of moderate halophilic bacteria consortium capable of degrading high-salinity benzene series and application of moderate halophilic bacteria consortium

A technology of moderate halophilic bacteria and construction method, which is applied in the construction and application field of moderate halophilic bacteria to achieve strong tolerance, broad application prospects and high degradation rate

Inactive Publication Date: 2012-11-14
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no existing literature about the function of the bacteria screened by the present invention to efficiently degrade toluene under high-salt conditions, and to use toluene as the only carbon source and energy source for growth

Method used

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  • Construction method of moderate halophilic bacteria consortium capable of degrading high-salinity benzene series and application of moderate halophilic bacteria consortium
  • Construction method of moderate halophilic bacteria consortium capable of degrading high-salinity benzene series and application of moderate halophilic bacteria consortium
  • Construction method of moderate halophilic bacteria consortium capable of degrading high-salinity benzene series and application of moderate halophilic bacteria consortium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1. Acclimatization and enrichment culture of moderately halophilic flora

[0022] a. Use a clean and sterilized shovel to collect soil samples with muddy water 5-10 cm below the surface, then quickly put them into a sterile ziplock bag, leaving some air in the upper part, bring the ziplock bag back to the laboratory and place it in 4 Store in a refrigerator at °C.

[0023] b. Add 40 ml of basic inorganic salt (MSM) medium with salt concentrations of 1 M, 1.5 M, 2 M, 2.5 M, 3 M, and 3.5 M NaCl, 500 ul of trace elements and 500 ul of vitamins into a 125 ml serum bottle. The composition of MSM medium includes:

[0024]

[0025] The trace element composition includes:

[0026] MnCl 2 4H 2 o 0.1 CoCl 2 ·6H 2 o 0.19 SnCl 2 0.07 h 3 BO 3 0.03 NiCl 2 ·6H 2 o 0.024 Na 2 MoO 4 2H 2 o 0.036 CuCl 2 2H 2 o 0.002 25% HCl (7.7M) 10mL Distilled water (1L) 990mL

[0027] Vitamin comp...

Embodiment 2

[0032] Example 2. Bacteria's ability to degrade toluene at different salinities

[0033] After obtaining the sludge-free bacterial liquid, the toluene-degrading ability of the bacterial flora acclimated at various salt concentrations of 1 M to 3.5 M was determined, as shown in figure 1 shown.

[0034] The flora can completely degrade 150 mg / L toluene within 7 days when the NaCl concentration is 1 M or 1.5 M. When the NaCl concentration was 2 M or 2.5 M, it took 9 days and 11 days to completely degrade 100 mg / L toluene, respectively. When the NaCl concentration is 3 M or 3.5 M, it takes a longer time, about 15 days, for the flora to completely degrade 100 mg / L of toluene. This result shows that the present invention has successfully acclimated and enriched the bacterial flora that can effectively degrade benzene series in a wide salinity range of 1 M to 3.5 M from soil samples.

[0035] A sterilized control sample was set up in the above experimental process, and the result...

Embodiment 3

[0036] Example 3. Continuous domestication of moderately halophilic flora

[0037] Among the flora obtained after 40 days of acclimatization, the culture medium was continuously acclimatized by continuously adding 100-120 mg / L of pure toluene at a salinity of 2 M NaCl (11.7%). During the acclimation process, take a small amount of culture fluid every other day to detect the degradation of the substrate toluene by gas chromatography. When the concentration of toluene in the culture fluid is lower than the detection limit of gas chromatography, add pure toluene again to a final concentration of 100-120mg / L. This process was repeated for more than 100 days, and the changes in the toluene degradation ability of the flora before and after domestication were as follows: figure 2 shown. It takes 9 days for the flora to completely degrade 100mg / L toluene at the beginning of acclimation, but at the end of acclimatization, the flora can completely degrade 120mg / L toluene within 5 days. ...

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Abstract

The invention discloses a construction method of a moderate halophilic bacteria consortium capable of efficiently degrading methylbenzene under a high-salinity condition and growing by using the methylbenzene as only carbon and energy sources. A group of moderate halophilic bacteria consortiums capable of degrading high-salinity benzene series is obtained through screening. The construction method comprises the following steps of: 1) inoculating a high-salinity soil sample polluted by the benzene series in a basic inorganic salt culture medium with NaCl concentration being 1M-3.5M, adding the methylbenzene as the only carbon and energy sources, and conducting aerobic culture under conditions of 30DEG C and 120r / min to obtain bacteria solution; and 2) continuously domesticating the bacteria consortium. The moderate halophilic bacteria consortium obtained by adopting the construction method can adapt to a wide salinity range and can degrade high-concentration methylbenzene by using the methylbenzene as the only carbon source. The moderate halophilic bacteria consortium not only can degrade the methylbenzene at very high speed, but also has a very strong capacity of tolerating high salinity and high methylbenzene concentration. Therefore, the moderate halophilic bacteria consortium has important research advantages and wide application prospect in the field of biological treatment of high-salinity benzene series wastewater.

Description

technical field [0001] The invention relates to the technical field of biological treatment of environmental pollutants, in particular to the construction and application of a moderately halophilic bacterial group that can efficiently degrade toluene under high-salt conditions and use toluene as the only carbon source and energy source for growth. Background technique [0002] Petroleum mining, printing and dyeing, tanning, pharmaceutical, chemical, food processing and other industries discharge a large amount of high-salt organic wastewater, generally with a salt content of about 15%-25%, and containing a variety of refractory or toxic organic substances. In recent years, the discharge of high-salt organic wastewater has shown a rapid growth trend. Half of the industrial wastewater discharged in my country is high-salt wastewater, and the annual discharge has exceeded 10 billion tons, which is equivalent to the water storage of a Taihu Lake. Among them, a large amount of ch...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20A62D3/02C02F3/34C12R1/01A62D101/20C02F101/32
Inventor 李辉刘勇弟林匡飞吕树光张倩陆强黄中子王玉凤王晓丽
Owner EAST CHINA UNIV OF SCI & TECH
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