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Vaccine for preventing decayed tooth and preparation method thereof

A technology for vaccines and dental caries, which is applied in the field of medicine and biology, can solve problems such as application limitations, and achieve the effects of simple preparation methods, easy purification, and significant economic benefits

Active Publication Date: 2012-11-14
CHENGDU OLYMVAX BIOPHARM +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cholera toxin (CT) and Escherichia coli heat-labile enterotoxin (LT) have been proved to have strong mucosal immune adjuvant effects, but their application is limited due to their toxicity

Method used

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  • Vaccine for preventing decayed tooth and preparation method thereof
  • Vaccine for preventing decayed tooth and preparation method thereof
  • Vaccine for preventing decayed tooth and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1, preparation prevents the antigenic protein of the vaccine of dental caries caused by S.Mutans infection

[0065] 1. PCR amplification of smu_862 DNA

[0066] 1. Primer design and synthesis

[0067] Primers were designed based on the "smu_862" nucleic acid sequence (812323..813030) in the S.mutans UA159 genome (GenBank: AE014133.1) published by GenBank. The 5' end of the upstream primer was introduced into the Sma I restriction site, and the 5' end of the downstream primer was introduced into NotI restriction site.

[0068] Primers were designed as follows:

[0069] smu_862-F 5'-TCC GCTATTTTGATAGTATTAGGCC-3'

[0070] Sma I restriction site

[0071] smu_862-R 5'-TTTTCCTTTT TCACCTCTTTTCATTAGCCTTA-3'

[0072] Not I restriction site

[0073] Primers were synthesized by Sangon Bioengineering (Shanghai) Co., Ltd.

[0074] 2. PCR amplification of target DNA

[0075] 1) Preparation of template

[0076] S. mutans UA159 (purchased from American Type Cu...

Embodiment 2

[0111] Example 2. Preparation of vaccines for preventing dental caries caused by S.mutans infection

[0112] 1. Preparation of LT S63K

[0113] (1) Whole gene synthesis LT S63K Gene

[0114] Synthesized by Sangon Bioengineering (Shanghai) Co., Ltd. LT S63K The gene sequence is shown as sequence 4 in the sequence table, wherein the 1st to 6th positions from the 5' end are the Nde I restriction site sequence, the 7th to 60th are the LTA signal peptide sequence, and the 61st to 783rd are LT S63K A gene sequence, the 780th to 842nd position is the LTB signal peptide sequence, the 843rd to 842nd position is the LTB gene sequence, and the 1155th to 1160th position is the BamH I restriction site sequence (see sequence 4 in the sequence listing).

[0115] (2) Recombinant plasmid pET11c / LT S63K Construct

[0116] 1. Digestion

[0117] LT S63K The gene and pET11c plasmid (NOVAGEN, Code: 69438-3) were digested with restriction endonucleases Nde I (TaKaRa, Code: D1161A) and BamH...

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Abstract

The invention discloses a vaccine for preventing decayed tooth and a preparation method thereof. Active ingredients of the vaccine for preventing a decayed tooth are proteins shown as 1) or 2) or 3): wherein 1) a protein is composed of amino acid sequences shown as a sequence 2 in a sequence table1; 2) a protein is derived by 1), relative to the prevention of the decayed tooth and formed by the fact that one or more amino acids is / are substituted and / or lost and / or added on amino acid sequences of the sequence 2 in the sequence table; and 3) a protein is composed of amino acid sequences shown as 9th position to 419th position of the sequence 2 in the sequence table. The vaccine has the advantages that the vaccine has a solubility expression and is easy to purify, the purity is high, the preparation method is simple and convenient and the like, and besides, the vaccine has remarkable economic benefits and can be used as a candidate vaccine for preventing the decayed tooth caused by streptococcal infections.

Description

technical field [0001] The invention relates to the field of medical biotechnology, in particular to a vaccine for preventing dental caries and a preparation method thereof. Background technique [0002] Dental caries is one of the most common human diseases, with a high incidence rate, and WHO ranks it as one of the three major human disease prevention and treatment together with cancer and cardiovascular disease (Petersen PE.Community Dent Oral Epidemiol.2003,31Suppl 1:3-23. ). In the latest "Global Oral Health Report" released by the World Health Organization, it is estimated that 5 billion of the world's 6.3 billion people have suffered from dental caries (Daniel J S.2010,9(1):1-3), and 60% in developed countries~ 90% of school-age children and most adults have suffered from dental caries. In my country, the incidence rate of adults is 50%, and that of children is as high as 70% to 90%. It has become a global problem that threatens human oral health. [0003] Dental car...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/09A61K39/39A61P1/02A61P31/04C12N15/31C12N15/70C07K14/315
Inventor 邹全明刘开云张卫军郭刚张怡孙红武解庆华樊绍文卢陆
Owner CHENGDU OLYMVAX BIOPHARM
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