Psychotria sp. extract, and preparation method and antineoplastic application thereof
A technology of Shandayan and its extracts, which is applied in the field of natural medicine and chemical medicine, can solve the problems that have not yet been reported on the anti-tumor research of Shandayan, and achieve the effects of good medicinal prospects, extensive resources, and easy large-scale production
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Embodiment 1
[0020] Embodiment 1, the preparation of Shandayan extract
[0021] Dry 2.0kg of the stem of Psychotria sp. (produced in Conghua, Guangdong), crush it and pass it through a 20-mesh sieve, use 25L of 95% ethanol to filter and extract, filter the extract, concentrate under reduced pressure to extract (300g), Then add 2L of water, carry out macroporous resin enrichment, eluent methanol-water gradient elution (10 percentage points is a gradient), TLC tracking, 50~90% section collection, decompression concentration to remove solvent is Shandayan Extract (60g). As determined by HPLC, the mass content of total triterpene saponins was 88%. Wherein the content of 7 kinds of triterpene saponins is successively: 8.5% of 1, 22.8% of 2, 2.5% of 3, 6.0% of 4, 2.5% of 5, 5.0% of 6, 15.0% of 7; mass percentage.
Embodiment 2
[0022] Embodiment 2, the preparation of Shandayan extract
[0023] Dry 2.0kg of the stem of Psychotria sp. (produced in Conghua, Guangdong), crush it and pass it through a 20-mesh sieve, use 25L of methanol to filter and extract, filter the extract, concentrate under reduced pressure to extract (350g), and then add 2L of water, for macroporous resin enrichment, eluent methanol-water (10 percentage points as a gradient), TLC tracking, 65~90% section collection, decompression concentration to remove solvent is Shandayan extract (70g) . As determined by HPLC, the mass content of total triterpene saponins was 93%. Wherein the content of 7 kinds of triterpene saponins is successively: 9.0% of 1, 35.0% of 2, 2.5% of 3, 7.0% of 4, 2.5% of 5, 5.5% of 6, 14.0% of 7; mass percentage.
Embodiment 3
[0024] Embodiment 3, the preparation of Shandayan extract
[0025] Dry 2.0kg of the stem of Psychotria sp. (produced in Conghua, Guangdong), crush it and pass it through a 20-mesh sieve, heat and reflux with 60-80% methanol / water 25L to extract, filter the extract, concentrate under reduced pressure to extract (500g), then add 2L of water to enrich the macroporous resin, eluent methanol-water (10% is a gradient), TLC tracking, 55~90% section collection, decompression concentration to remove the solvent is Shanda Yan Extract (75g). As determined by HPLC, the mass content of total triterpene saponins was 95%. Wherein the content of 7 kinds of triterpene saponins is successively: 8.0% of 1, 37.0% of 2, 3.0% of 3, 7.0% of 4, 3.0% of 5, 4.5% of 6, 13.5% of 7; the percentage is mass percentage.
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