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Application of teprenone in prevention and treatment of morphine-induced liver injury

A technology for teprenone and liver damage, applied in the field of medicine, to achieve high safety, reduce economic burden, and significant curative effect

Inactive Publication Date: 2012-11-07
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Whether teprenone can be used to prevent and treat liver injury caused by morphine has not been reported

Method used

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  • Application of teprenone in prevention and treatment of morphine-induced liver injury
  • Application of teprenone in prevention and treatment of morphine-induced liver injury
  • Application of teprenone in prevention and treatment of morphine-induced liver injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Teprenone inhibits morphine-induced activation of caspase-9 in the liver

[0025] Mice were divided into 4 groups (6 mice in each group): control group (saline), morphine group (Mor), GGA and morphine group (GGA+Mor), and GGA group. From day 1 to day 7, Saline group and Mor group were pre-administered with normal saline, GGA+Mor group and GGA group were pre-administered with GGA (800 mg / kg), 2 hours later, Saline group and GGA group were intraperitoneally injected Normal saline, Mor group and GGA+Mor group received intraperitoneal injection of morphine (daily injection dose: 10, 20, 40, 60, 80, 100 and 100 mg / kg). Two hours after the last morphine injection, the liver tissue of the mice was isolated, the protein was extracted, and the expression of pro-caspase-9 in the liver cells was detected by Western blotting. figure 1 The results showed that pre-infusion of GGA could inhibit the reduction of pro-caspase-9 induced by morphine, which indicated that tepr...

Embodiment 2

[0026] Example 2: Teprenone inhibits morphine-induced activation of caspase-3 in the liver

[0027] Mice were divided into 4 groups (6 mice in each group): control group (saline), morphine group (Mor), GGA and morphine group (GGA+Mor), and GGA group. From day 1 to day 7, Saline group and Mor group were pre-administered with normal saline, GGA+Mor group and GGA group were pre-administered with GGA (800 mg / kg), 2 hours later, Saline group and GGA group were injected intraperitoneally Normal saline, Mor group and GGA+Mor group received intraperitoneal injection of morphine (daily injection dose: 10, 20, 40, 60, 80, 100 and 100 mg / kg). Two hours after the last morphine injection, the liver tissue of the mice was isolated, the protein was extracted, and the expression of caspase-3 in liver cells was detected by Western blotting. figure 2 The results showed that pre-infusion of GGA could inhibit the increase of active caspase-3 induced by morphine, which indicated that teprenone c...

Embodiment 3

[0029] Example 3: Teprenone inhibits morphine-induced increases in malondialdehyde (MDA) in the liver

[0030] Mice were divided into 4 groups (6 mice in each group): control group (saline), morphine group (Mor), GGA and morphine group (GGA+Mor), and GGA group. From day 1 to day 7, Saline group and Mor group were pre-administered with normal saline, GGA+Mor group and GGA group were pre-administered with GGA (800 mg / kg), 2 hours later, Saline group and GGA group were injected intraperitoneally Normal saline, Mor group and GGA+Mor group received intraperitoneal injection of morphine (daily injection dose: 10, 20, 40, 60, 80, 100 and 100 mg / kg). Two hours after the last morphine injection, the liver tissue of the mice was separated, prepared into a 10% homogenate with phosphate buffer in an ice bath, and centrifuged in a high-speed refrigerated centrifuge for 10 minutes (4°C, 4000 rpm). The supernatant was taken, and the content of malondialdehyde (MDA) was detected using malo...

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Abstract

The invention relates to a new application of teprenone in preparation of a medicament for preventing and treating morphine-induced liver injury, and discloses teprenone capable of protecting liver cells from being poisoned by morphine, inhibiting morphine-induced cell apoptosis and weakening morphine-induced lipid per-oxidative injury. The teprenone can be used for preventing and treating the morphine-induced liver injury.

Description

technical field [0001] The invention relates to a new application of teprenone, in particular to the application of teprenone in the preparation of medicines for preventing liver damage caused by morphine, and belongs to the technical field of medicine. Background technique [0002] The abuse of opioids has become a serious social problem at home and abroad. Morphine, a powerful analgesic commonly used to treat acute and chronic pain, is a type of opioid. However, long-term use of morphine can lead to cell death and apoptosis in multiple systems and organs, and its clinical application is greatly limited (Nestler EJ, Aghajanian GK. Molecular and cellular basis of addiction. Science. 1997; 278: 58-63. Yin D, Mufson RA, Wang R, Shi Y. Fas- [0003] Mediated cell death promoted by opioids. Nature. 1999; 397-218. Hu S, Sheng WS, Lokensgard JR, Peterson PK. Morphine induces apoptosis of human microglia and neurons. Neuropharmacology. 2002; 42:829—836.). Among them, the damage ...

Claims

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Application Information

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IPC IPC(8): A61K31/121A61P1/16
Inventor 白洁罗富成梁敏赵璐
Owner KUNMING UNIV OF SCI & TECH
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