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Production method and culturing device of high-vigor nitrifying bacteria products

A technology of nitrifying bacteria and production methods, applied in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve high production costs, unsuitable for large-scale production, and heavy workload of elution and collection and other issues to achieve the effect of saving energy

Inactive Publication Date: 2012-10-31
NINGBO UNIV
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Problems solved by technology

[0002] Nitrifying bacteria are a kind of autotrophic aerobic bacteria, including ammonia oxidizing bacteria and nitrous acid oxidizing bacteria, which play an important role in nitrogen cycle and water purification process. Because nitrifying bacteria reproduce slowly, they do not follow the law of separation and freedom Combination law, the survival of nitrifying bacteria needs water and high oxygen, so they can only live in various microporous filter materials such as biochemical cotton, biochemical balls, glass rings, ceramic rings, etc. Nitrifying bacteria are most suitable in Living in weakly alkaline water, the growth and reproduction is the fastest when the temperature reaches about 25 degrees Celsius, so the cultivation and production of nitrifying bacteria are relatively difficult
At present, nitrifying bacteria are mainly cultivated in natural water bodies. When the content of nitrifying bacteria in water is low, measures such as oxygen supply and pH adjustment cannot make nitrifying bacteria grow naturally in a short period of time. The production cost is high, and the production cost is high. Patent document CN200310112485.5 discloses a production method of arranging soft fibers in an aerobic fermenter to collect nitrifying bacteria, although soft fibers are beneficial to nitrifying bacteria Attached growth, but the workload of elution and collection is heavy, not suitable for large-scale production

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  • Production method and culturing device of high-vigor nitrifying bacteria products

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Embodiment Construction

[0014] Below in conjunction with accompanying drawing, embodiment the invention is further described.

[0015] Such as figure 1 Shown is a culture device for cultivating high-activity nitrifying bacteria products, including a tank body 1 made of heat-insulating material, with a temperature control device in the tank body to create temperature conditions for cultivating nitrifying bacteria, and a cover 2 fixed on the tank body 1 through a flange 3 The upper part of the upper part of the lid 2 is provided with a four-way pipe 4, one of the pipes of the four-way pipe 4 is inserted into the tank body, and the nutrient solution is delivered to the inside of the tank body 1 through the other three pipes, and the three pipes are respectively used to transport three kinds of different components. The nutrient solution prevents the nutrient solution from reacting and precipitation before mixing, and the three pipelines are provided with a liquid filter 5 and a valve 6, and the liquid f...

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Abstract

The invention discloses a production method and a culturing device of high-vigor nitrifying bacteria products. The production method comprises the steps of: placing an adsorption device used for growth and adsorption of nitrifying bacteria in the culturing device, wherein the total volume of the adsorption device takes up 20%-30% of the effective volume of a container; adding a culture medium in the culturing device; boiling; adding a nitrifying bacteria seed liquid; opening an aerating system and a temperature control system; dividing the culture medium into three different components after culturing continuously for 120 h; respectively inputting the three components into a culturing container through a four-way tube; controlling hydraulic retention time (HRT) to be equal to 30 h; taking out the adsorption device and drying the nitrifying bacteria on the adsorption device to form finished products; then placing a new adsorption device into the culturing container and then circulating to perform flowing culture and drying collection on the nitrifying bacteria; and then replenishing nitrifying bacteria strains in the culturing container after multiple circulations. The production method can be used to culture two nitrifying bacteria at the same time and is favorable for solving the problems of product suppression, precipitation formation under interaction of culture media, bacteria removal of a filtering membrane and the like.

Description

technical field [0001] The invention relates to a production method and production equipment of nitrifying bacteria, in particular to a production method and a culture device of high-activity nitrifying bacteria. Background technique [0002] Nitrifying bacteria are a kind of autotrophic aerobic bacteria, including ammonia oxidizing bacteria and nitrous acid oxidizing bacteria, which play an important role in the process of nitrogen cycle and water purification. Due to the slow reproduction of nitrifying bacteria, they do not follow the law of separation and freedom Combination law, the survival of nitrifying bacteria needs water and high oxygen, so they can only live in various microporous filter materials such as biochemical cotton, biochemical balls, glass rings, ceramic rings, etc. Nitrifying bacteria are most suitable in Living in weakly alkaline water, the growth and reproduction is the fastest when the temperature reaches about 25 degrees Celsius, so the cultivation a...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12M1/00
CPCC12M41/12C12M47/04C12M45/03
Inventor 张德民章文军王一农潘志崇
Owner NINGBO UNIV
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