Sulfur-substituted podophyllum derivative and bioconversion, separation and purification method thereof
A technology of separation, purification and biotransformation, which is applied in the fields of content detection, preparation of antineoplastic drugs, sulfur-substituted podophyllum derivatives and their biotransformation, separation and purification, podophyllum compounds and their preparation, and can solve the problem of high prices and production problems. High cost, small scale and other problems, to achieve the effect of simple operation, low cost and short cycle
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Embodiment 1
[0084] 1. Podophyllotoxin is converted into 4-(1,2,4-triazole-3)-4-deoxy-podophyllotoxin and 4-S-(1,3,4-thiadiazole-2)-4 - Deoxypodophyllotoxin
[0085] 1), slant strain cultivation: the formula of the slant strain culture medium adopted in the present embodiment is (nutritional PDA slant): glucose 30 grams / liter, magnesium sulfate 0.5 grams / liter, potassium dihydrogen phosphate 1.5 grams / liter, Thiamine hydrochloride 0.1 g / L, agar 20 g / L, potato extract 1.0 L; the preparation method of potato dipping juice: 200g fresh potatoes are peeled and cut into cubes with a side length of about 1cm, add 1L of deionized water, Boil for 15 minutes, filter with eight layers of gauze, take the filtrate and dilute it to 1L again, and the obtained solution is used to prepare PDA medium.
[0086] Penicillium purpurogenum Y.J.Tang was inoculated on the PDA slant by the two-point method with an inoculation needle. The culture conditions: the culture temperature was 30°C. Sterilization conditio...
Embodiment 2
[0102] 1, 4'-desmethyl epipodophyllotoxin is converted into 4-S-(1,2,4-triazole-3)-4-deoxy-4'-desmethyl epipodophyllotoxin and 4-S -(1,3,4-Thiadiazole-2)-4-deoxy-4′-desmethylepipodophyllotoxin
[0103] 1), slant strain cultivation: the formula of the slant strain culture medium adopted in the present embodiment is (nutritional PDA slant): glucose 30 grams / liter, magnesium sulfate 0.5 grams / liter, potassium dihydrogen phosphate 1.5 grams / liter, Thiamine hydrochloride 0.1 g / L, agar 20 g / L, potato extract 1.0 L; the preparation method of potato dipping juice: 200g fresh potatoes are peeled and cut into cubes with a side length of about 1cm, add 1L of deionized water, Boil for 15 minutes, filter with eight layers of gauze, take the filtrate and dilute it to 1L again, and the obtained solution is used to prepare PDA medium.
[0104] Penicillium violet-producing Y.J.Tang was inoculated on the PDA slant by the two-point method with an inoculation needle. The culture conditions: the ...
Embodiment 3
[0121] 1. 4-S-(1,2,4-triazole-3)-4-deoxy-4'-demethyl epipodophyllotoxin is converted into 4-S-(1,2,4-triazole -3)-4-Deoxy-4'-desmethyl epipodophyllic acid (compound (5))
[0122]1), slant strain cultivation: the formula of the slant strain culture medium adopted in the present embodiment is (nutritional PDA slant): glucose 30 grams / liter, magnesium sulfate 0.5 grams / liter, potassium dihydrogen phosphate 1.5 grams / liter, Thiamine hydrochloride 0.1 g / L, agar 20 g / L, potato extract 1.0 L; the preparation method of potato dipping juice: 200g fresh potatoes are peeled and cut into cubes with a side length of about 1cm, add 1L of deionized water, Boil for 15 minutes, filter with eight layers of gauze, take the filtrate and dilute it to 1L again, and the obtained solution is used to prepare PDA medium.
[0123] Gibberella Fujikura SH-f13 was inoculated on the PDA slant by the two-point method with an inoculation needle, and the culture conditions were as follows: the culture tempera...
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