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Extracellular matrix support material and preparation method thereof

A scaffold material and extracellular matrix technology, applied in the extracellular matrix scaffold material and its preparation, based on the extracellular matrix scaffold material derived from bone marrow mesenchymal stem cells and the field of preparation, can solve the problem of aminoprotein sugar loss of collagen and loss of growth factors , tissue structure fracture and other problems, to achieve the effect of strong proliferation ability and wide tissue source

Inactive Publication Date: 2012-10-24
金成哲 +3
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AI Technical Summary

Problems solved by technology

The protein denaturant and co-solvent SDS used in the decellularization process will not only break the normal tissue structure, lose the amino protein sugar and destroy the collagen, but also lose a large amount of growth factors (up to 39-72%), thereby reducing the cell growth rate. Biological functions of extracellular matrix (ECM) scaffolds

Method used

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  • Extracellular matrix support material and preparation method thereof
  • Extracellular matrix support material and preparation method thereof
  • Extracellular matrix support material and preparation method thereof

Examples

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Embodiment 1

[0032] (1) The schematic diagram of the preparation process of the extracellular matrix scaffolds of bone marrow mesenchymal stem cells is shown in figure 1 , the specific method is as follows:

[0033] (1) Primary culture of young rabbit bone marrow mesenchymal stem cells by whole bone marrow culture method: Take fresh 3-week-old young rabbits just slaughtered in the slaughterhouse, separate left and right femurs and tibias, put them in ice packs and transport them back to the laboratory for the following operations : Sterilize the femur and tibia by immersion in iodophor, and place them in a petri dish with sterile normal saline. In the ultra-clean table, open the bone marrow cavity at both ends of the femur and tibia, and flush the marrow cavity with normal saline until the marrow cavity turns white. The bone marrow washing fluid was collected, centrifuged at 1000 rpm for 10 minutes, the supernatant was discarded, the cell pellet was suspended in DMEM medium, and counted. ...

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Abstract

The invention belongs to the field of biological materials and discloses an extracellular matrix support material and a preparation method thereof. The extracellular matrix support material disclosed by the invention is prepared into a three-dimensional porous support material with a certain pore diameter and porosity through performing in-vitro monolayer high-density culture on immature rabbit bone marrow mesenchymal stem cells and collecting extracellular matrixes secreted by the immature rabbit bone marrow mesenchymal stem cells by applying the freeze-drying technology and a proper cross-linking agent. The material can be trimmed into proper sizes and shapes according to a cartilage defect zone or the requirement of an in-vitro experiment, and the material is sterilized by ethylene oxide to obtain an integral packaging bag. As the stem cells are used as material sources of an extracellular matrix support, the extracellular matrix support material disclosed by the invention is wide in tissue source and strong in multiplication capacity, therefore, the extracellular matrix support material can obtain enough cell quantity per se, and the problems of social ethics involved by embryonic stem cells are avoided.

Description

technical field [0001] The invention belongs to the field of biological materials, and relates to an extracellular matrix scaffold material and a preparation method thereof, in particular to an extracellular matrix scaffold material derived from bone marrow mesenchymal stem cells and a preparation method thereof. Background technique [0002] Articular cartilage is a special tissue without blood vessels, lymphatics and innervation. The articular cartilage itself has a very limited ability to repair itself, and once the damaged diameter is greater than 3mm, it will not be able to repair itself. At present, the main methods for the treatment of cartilage defects are: arthroscopic lavage, joint debridement, subchondralbone drilling, microfracture, auto or allocartilage transplantation (auto or allo osteochondral). transplantation), autologous chondrocyte implantation (ACI), and artificial joint replacement. Each of the above methods has surgical indications and advantages and...

Claims

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Application Information

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IPC IPC(8): A61L27/36C12N5/0775
Inventor 金成哲徐燕唐成王黎明
Owner 金成哲
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