Visualized detection probes and application thereof in tuberculosis rifampicin resistance mutation detection

Abstract

The invention discloses novel visualized fast and sensitive detection probes and applications thereof in mycobacterium tuberculosis rifampicin resistance mutation detections. The visualized probes provided by the invention comprise: a common anchoring probe A, insulation probes L and R, mutation detection probes B, and a common competition probe SIB. The mutation detection probes B and the probe SIB have a same target DNA complementary region, and only the base types of the mutation sites are different. Aims at rifampicin resistance mutation, a detection probe cluster is designed and synthesized according to all the mutation sites included in a mycobacterium tuberculosis rifampicin resistance rpoB genetic mutation core. The probe cluster comprises 8 probe sets, wherein each probe set comprises a specific mutation detection probe B. With the method provided by the invention, the designed probe sets and probe clusters have good distinguishing ability upon synthesized wild-type and mutant detection fragments, and can be used for accurately detecting specific mutation sites of 530-533 site amino acids of the mycobacterium tuberculosis rifampicin resistance rpoB gene.

Description

technical field [0001] The invention belongs to the fields of pathogenic biology, molecular microbiology and nucleic acid chemistry, and relates to a design method and sequence of a novel gene point mutation detection probe, and its application in the detection of rifampicin-resistant mutations of Mycobacterium tuberculosis. This method can intuitively, rapidly and visually detect rifampicin-resistant mutations in Mycobacterium tuberculosis. Background technique [0002] Tuberculosis (TB for short) caused by Mycobacterium tuberculosis is an ancient disease that has been widely spread in the world long ago. Since the 1950s, effective drugs such as isoniazid and rifampicin have been discovered for the treatment of tuberculosis. , greatly improved the cure rate of tuberculosis, and reduced the recurrence rate of tuberculosis, making tuberculosis a curable and controllable disease. In some developed countries, tuberculosis has been largely brought under control. However, in re...

AI Technical Summary

Problems solved by technology

The methods currently used by researchers to detect drug-resistant mutations in Mycobacterium tuberculosis include molecular beacons and reverse probe hybridization, but these two methods are more complicated to operate and require specialized instruments

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