Thermophilic facultative aerobic bacteria UTM601 and application thereof
A technology of facultative bacteria and strains, applied in the field of environmental microorganisms, can solve the problem of no public report of thermophilic facultative bacteria, and achieve the effects of avoiding ventilation management control problems, low cost and simple operation.
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Embodiment 1
[0031] Example 1 Isolation and identification of thermophilic facultative bacteria UTM601
[0032]Weigh 10g of high-temperature period sludge compost sample (collected from the compost fermentation workshop of Dadi Lvyuan Environmental Protection Technology (Beijing) Co., Ltd.) into a 250ml triangular flask filled with 10 glass beads and 90ml sterile water, and shake it on a shaker at 200rpm 30 minutes, then take it out and let it stand for 30 minutes, take 10ml supernatant and connect to 90ml NA culture solution (beef extract 4g, peptone 8g, sodium chloride 4g, agar 16g, plant gel 8g, distilled water 1000ml, pH7.2) Placed on a water bath shaker, at 60°C, variable speed acclimation culture: every 6 hours as a time unit, with 50rpm as a variable speed unit, gradually increase the speed until it reaches 150rpm, then gradually reduce the speed, which is One acclimatization cycle; after completing 3 acclimatization cycles, take 10ml of acclimatization liquid and transfer it to new...
Embodiment 2
[0038] The preparation of embodiment 2 compost inoculants (1)
[0039] (1) Preparation of activation solution: take 1 slant surface stored at 4°C of the microorganism UTM601 of the present invention and inoculate it into 5 test tubes containing 10ml of sterilized NA liquid culture solution, and cultivate it in a constant temperature water bath at 55°C for 1.5 days to obtain slant activation. bacteria liquid. OD of activated bacterial solution on inclined plane 600 is 0.8.
[0040] (2) Preparation of seed solution: Inoculate one test tube of the above-mentioned slant-activated bacterial solution into 1L of sterile NA liquid culture solution, culture on a water-bath shaker with shaking at a temperature of 55°C, and an oscillation frequency of 50rpm, and cultivate for 3 days to obtain a seed solution. OD of seed solution 600 is 1.2.
[0041] (3) Preparation of fermented seed liquid: the above seed liquid was inoculated into a sterilized fermenter at an inoculum amount of 5% (...
Embodiment 3
[0043] The preparation of embodiment 3 compost inoculants (2)
[0044] (1) Prepare the activation solution, and the culture conditions are: take 1 slant stored at 4°C of the microorganism UTM601 of the present invention and inoculate it into 5 sterilized NA liquid culture medium test tubes containing 10ml, and cultivate it in a constant temperature water bath at 50°C for 2 days , the OD of the activated bacterial solution on the inclined plane 600 is 1.2.
[0045] (2) Prepare the seed liquid, cultivate the temperature at 50°C, shake the frequency at 160rpm, and obtain the seed liquid after culturing for 4 days, the OD of the seed liquid 600 is 1.5.
[0046] (3) In the preparation of the fermented seed liquid, the inoculation amount of the above seed liquid is 15% (v / v), and the conditions for the expanded fermentation culture are: temperature 50°C, ventilation for 20 minutes at intervals of 5 hours, volume ratio of ventilation volume 0.3:1 , stirred at 160rpm, and cultivate...
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