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Mixed microbial inoculant generating biogas at low temperature in Northeast of China, and preparation method thereof

A technology of mixing microorganisms and microbial inoculants, applied in the field of microbial inoculants, can solve problems such as difficult gas production

Inactive Publication Date: 2012-10-17
张建峰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problem of low temperature and difficult gas production that currently restricts the development of biogas digesters in Northeast my country, the present invention provides an excellent mixed microbial bacterial agent and its preparation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Trichoderma viride, Bacillus subtilis, Pseudomonas putida, Bacillus licheniformis and Alcaligenes were purchased from China Microorganism Culture Collection. Use conventional methods to separately culture 5 kinds of bacteria to obtain 5 kinds of bacterial liquids for use, and the bacterial liquids are required to contain 1 billion active bacteria / ml.

[0040] The specific method is as follows:

[0041] (1) Bacteria activation: 5 kinds of bacteria were activated according to the conventional method, and the culture temperature was 23-28°C.

[0042] (2) Shake flask culture: Introduce the activated strains into the liquid medium respectively. Trichoderma viride uses: bran 5.0g, rice bran 12.0g, (NH 4 ) 2 SO 4 2.0g, KH 2 PO 4 1.0g, CaCl 2 1.0g, 6.0g glucose, 1000mL tap water, pH5.3-3.7. Bacillus subtilis uses: beef extract 3g, peptone 10g, NaCl 5g, tap water 1000mL, pH7.2-7.4. Pseudomonas putida uses: beef extract 3g, peptone 10g, NaCl 5g, tap water 1000mL, pH7.2...

Embodiment 2

[0047] The preparation of the liquid mixed microbial bacterial agent is as in Example 1.

[0048] 1 part of peat or 1 part of wheat bran is sterilized and mixed evenly with 0.15 part of liquid mixed microbial inoculum to obtain a solid mixed microbial inoculum.

Embodiment 3

[0050] The preparation of the liquid mixed microbial bacterial agent is as in Example 1.

[0051] The liquid mixed microbial bacterial agent is added with 5% dry matter weight skimmed milk powder as a drying protection agent, vacuum-low-temperature dehydration and drying to make a dormant dry powder, which is tested and packaged.

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Abstract

The invention relates to a mixed microbial inoculant suitable for biogas digesters to generate biogas under low temperature conditions in the Northeast of China. The mixed microbial inoculant is obtained by mixing 1.4-1.8 parts of a fermentation broth of Trichoderma viride, 1.8-2.3 parts of a fermentation broth of Bacillus subtilis, 4.1-6.5 parts of a fermentation broth of Pseudomonas putida, 1.1-1.5 parts of a fermentation broth of Bacillus licheniformis and 0.1-0.2 parts of a fermentation broth of Alcaligenes. The mixed microbial inoculant is adopted to make most biogas digesters in the Northeast of China be normally used in winter, and biogas digesters stopped in early winter be started. The invention relates to a preparation method of the mixed microbial inoculant generating the biogas at a low temperature in the Northeast of China.

Description

technical field [0001] The invention belongs to the technical field of microbial bacterial agents in biogas technology, and in particular relates to a biogas produced by fermenting chicken manure, pig manure, straw, etc. in the cold region of Northeast China, which solves the problems of difficult feces fermentation in biogas pits and difficult biogas production at low temperature in winter. Background technique [0002] At present, the construction of rural biogas digesters has become an important part of building a new socialist countryside. Using biogas as energy can not only save a lot of oil and coal consumption, but also reduce the economic burden of farmers. At the same time, the use of rural biogas digesters can also eliminate livestock and poultry manure pollution and reduce environmental pollution caused by straw burning. Biogas residue is also an excellent organic fertilizer. [0003] At present, there are some biogas digesters built in Northeast China, most of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12P5/02C02F11/04C12R1/885C12R1/125C12R1/40C12R1/10C12R1/05
CPCY02E50/343Y02E50/30
Inventor 张建峰
Owner 张建峰
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