Extraction method of salvia miltiorrhiza polysaccharide from tanshinol residues
A technology of salvia miltiorrhiza alcohol and residue, which is applied in the field of extracting salvia miltiorrhiza polysaccharides from the alcohol extraction residue of salvia miltiorrhiza, can solve the problem of low extraction rate of salvia miltiorrhiza polysaccharides, and achieve the effect of saving protein removal process, low polysaccharide loss rate and high purity
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[0023] 1. Materials and reagents
[0024] Alcohol extraction residue of Danshen; cellulase, pectinase, papain, absolute ethanol, concentrated sulfuric acid (excellent grade), glucose, phenol, 3-5-dinitrosalicylic acid, potassium sodium tartrate, unless otherwise specified Others are analytically pure.
[0025] 2. Experimental equipment
[0026] UV-visible spectrophotometer (Shimazu UV-1750), PHS-320 intelligent acidity meter, HH.S11-Ni6 electric heating constant temperature water bath, KQ-300GDV constant temperature numerical control ultrasonic cleaner, sartorius series electronic balance, GL-2050M high-speed freezing Centrifuge, DZ-2BC vacuum drying oven, RE-52AA / RE-52A rotary evaporator, etc.
[0027] 3 Experimental methods
[0028] 3.1 Technical route of polysaccharide extraction
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