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Multipath pre-activating device, peptide nucleic acid preparation system, and pre-activating and synthetizing method

A preparation system and pre-activation technology, applied in the field of biomacromolecule preparation, can solve the problems of affecting activation effect, reducing monomer coupling efficiency, uneven mixing of monomer and activator, etc., to improve coupling efficiency, facilitate cleaning, The effect of protecting personnel safety

Active Publication Date: 2014-06-11
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although PNA is an analogue of DNA and polypeptides, since PNA is different from DNA or polypeptides in molecular structure and chemical properties, pre-activation of monomers is required during the synthesis of PNAs, and the existing DNA or polypeptide synthesizers do not have a single The body preactivation device mainly controls the activation by the residence of the monomer and the activator in the pipeline. The uneven mixing of the monomer and the activator will affect the activation effect and directly reduce the coupling efficiency of the monomer.

Method used

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  • Multipath pre-activating device, peptide nucleic acid preparation system, and pre-activating and synthetizing method
  • Multipath pre-activating device, peptide nucleic acid preparation system, and pre-activating and synthetizing method
  • Multipath pre-activating device, peptide nucleic acid preparation system, and pre-activating and synthetizing method

Examples

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Effect test

Embodiment 1

[0058] Such as figure 1 , figure 2 , the present invention is made up of inert gas source 2, gas electromagnetic valve array 6, multi-channel preactivation device 13, fluid electromagnetic valve array 25 and PLC4; Described inert gas source 2 selects high-purity argon, helium or nitrogen, and The gas pipeline 3 is connected to the common air inlet 8 of the gas solenoid valve array to provide gas source power for reagent delivery;

[0059] The gas solenoid valve array 6 is arranged by a series of two-way or three-way solenoid valves, and the control terminal 7 of each solenoid valve in the array is connected with the control output terminal 5 of the PLC in turn, and the common progress of the array is The gas port 8 is connected to the inert gas source 2; the gas outlet 9 of one solenoid valve in the array is directly connected to the multi-channel preactivation device 19, and the gas outlet 9 of the other solenoid valves is connected to the reagent bottle 11, and the gas sol...

Embodiment 2

[0064] Combination of the working principles of the multi-channel preactivation device 13 of the peptide nucleic acid preparation system of the present invention figure 2 The following explanations are made: In this embodiment, the preactivated peptide nucleic acid monomer T is taken as an example. Under the control of an external computer command, the PLC performs the following specific operations according to the control output connection table in Attached Table 1: (1) PLC Open the control output terminal Y0, and open the air pressure balance switch 30 in the multi-channel pre-activation device. At this time, the multi-channel pre-activation device 13 is in the state of receiving reagents; (2) PLC simultaneously opens the control output terminals Y5, Y6 and Y7, and turns on The solenoid valves in the gas solenoid valve array are respectively connected to reagent bottles 11 containing peptide nucleic acid monomer T, alkali solution and activator; control the simultaneous deli...

Embodiment 3

[0066] This example takes 9-fluorenyloxycarbonyl (Fmoc) protected peptide nucleic acid monomer as an example to synthesize a single-stranded peptide nucleic acid 5'-ATCG-3', using such as Figure 4 The microsphere particles in a are used as synthetic carriers, and a large number of microsphere particles are placed in the reaction pool. Input the required synthetic peptide nucleic acid single-strand sequence into the external computer program, after the external computer program completes the necessary initialization, the external computer follows the Figure 8 The program flow shown sends a series of instructions to the PLC, and the PLC performs the following specific tasks according to the received instructions: (see Table 1 for the specific control objects of the PLC)

[0067] (1) Counter i=1, where i corresponds to the i-th position of the peptide nucleic acid sequence, such as i=2 corresponding to the peptide nucleic acid monomer is T;

[0068] (2) Deprotection reaction: ...

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Abstract

The invention discloses a multipath pre-activating device, a peptide nucleic acid preparation system, and a pre-activating and synthetizing method. The peptide nucleic acid preparation system comprises the multipath pre-activating device, and further comprises an inert gas source, a gas electromagnetic valve array, a fluid electromagnetic valve array, a PLC (programmable logic controller), a computer, a reaction tank and a plurality of reagent bottles. The synthetizing method comprises a method for synthetizing peptide nucleic acid single-chain and a method for synthetizing peptide nucleic acid arrays. The multipath pre-activating device can effectively perform quantifying, and periodically mixing and activating to monomer reagents, and increase coupling reaction efficiency; at the same time, the multipath pre-activating device can coordinate with the electromagnetic valve arrays to realize multi-flow-path liquid transferring control, and can be cleaned easily. The device, system and method provided by the invention can meet demand on synthesis of peptide nucleic acids, and can be used for synthesis of peptide nucleic acid single-chain and peptide nucleic acid arrays.

Description

technical field [0001] The invention belongs to the technical field of biomacromolecule preparation, and in particular relates to a multi-channel preactivation device, a peptide nucleic acid preparation system and a preactivation and synthesis method. Background technique [0002] Peptide nucleic acid (PNA) is an analog of DNA. The backbone of PNA is composed of N-(2-aminoethyl)glycine and nucleic acid bases connected by methylene carbonyl. PNA can specifically hybridize with DNA or RNA to form a stable complex. At the same time, PNA as a hybridization probe greatly improves the efficiency and sensitivity of genetic testing and medical diagnosis. [0003] At present, there is still no synthesis equipment for PNA in the domestic and foreign markets. The synthesis of PNA is similar to the synthesis of DNA and peptides. It needs to go through several cycles of deprotection reaction, coupling reaction, and capping reaction. Therefore, the preparation of existing PNA is mainly ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/00C07K14/00C40B50/06
Inventor 刘正春邬燕琪王宏银杨飞鹏
Owner CENT SOUTH UNIV
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