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Preservative agent for DNA of saliva

A technology of preservative and saliva, applied in the field of medical biology, can solve the problems of low DNA amount and short storage time, and achieve the effects of low cost, short storage time and simple preparation method.

Active Publication Date: 2012-09-19
XIAMEN ZEESAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the collection of saliva DNA samples is mainly carried out by using cotton swabs to obtain oral mucosal epithelial cells. The amount of DNA collected by this method is small, and it needs to be stored at low temperature, and the storage time is short.

Method used

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  • Preservative agent for DNA of saliva
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  • Preservative agent for DNA of saliva

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Preparation of saliva DNA preservation agent:

[0030] (1) Weigh each component according to the data in the table below, and completely dissolve disodium edetate to make a mother liquor.

[0031] (2) After adding other components in water and fully dissolving them, add the above mother liquor and mix thoroughly.

[0032] (3) After adjusting the pH with hydrochloric acid to the final pH value of the solution in the table below, add water to make up to the required volume.

[0033]

[0034] 2. The saliva DNA preservative prepared by the above formula was fully mixed with the collected four saliva samples at a volume ratio of 1:1 to obtain a mixed solution, and each 500 μL of the above mixed solution was extracted with a whole blood genomic DNA extraction kit (Xiamen Zhishan Biotechnology Co., Ltd. Produced by Science and Technology Co., Ltd. (Product No. 4hk021) to extract DNA and dissolve in 100 μL eluent to obtain four corresponding samples, respectively numbere...

Embodiment 2

[0042] 1. Preparation of saliva DNA preservation agent:

[0043] (1) Weigh each component according to the data in the table below, and completely dissolve disodium edetate to make a mother liquor.

[0044] (2) After adding other components in water and fully dissolving them, add the above mother liquor and mix thoroughly.

[0045] (3) After adjusting the pH with hydrochloric acid to the final pH value of the solution in the table below, add water to make up to the required volume.

[0046]

[0047]2. The saliva DNA preservative prepared by the above formula was fully mixed with the collected four saliva samples at a volume ratio of 1:1 to obtain a mixed solution, and each 500 μL of the above mixed solution was extracted with a whole blood genomic DNA extraction kit (Xiamen Zhishan Biotechnology Co., Ltd. Produced by Science and Technology Co., Ltd., product number 4hk021) to extract DNA, and dissolve in 100 μL eluent to obtain four corresponding samples, respectively numb...

Embodiment 3

[0055] 1. Preparation of saliva DNA preservation agent:

[0056] (1) Weigh each component according to the data in the table below, and completely dissolve disodium edetate to make a mother liquor.

[0057] (2) After adding other components in water and fully dissolving them, add the above mother liquor and mix thoroughly.

[0058] (3) After adjusting the pH with hydrochloric acid to the final pH value of the solution in the table below, add water to make up to the required volume.

[0059]

[0060] 2. The saliva DNA preservative prepared by the above formula was fully mixed with the collected four saliva samples at a volume ratio of 1:1 to obtain a mixed solution, and each 500 μL of the above mixed solution was extracted with a whole blood genomic DNA extraction kit (Xiamen Zhishan Biotechnology Co., Ltd. Produced by Science and Technology Co., Ltd. (Product No. 4hk021) to extract DNA and dissolve in 100 μL of eluent to obtain four corresponding samples, respectively numb...

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Abstract

The invention discloses a preservative agent for DNA (Deoxyribose Nucleic Acid) of saliva. The preservative agent comprises the following components in percentage by volume: 5-10 g / 100mL of trihytdroxy methyl-aminomethane, 10-25 g / 100mL of ethylene diamine tetraacetic acid, 7-17 g / 100mL of sugar, 1-10 g / 100mL of sodium chloride and 0.5-5 g / 100mL of surfactant. The solvent of the preservative agent is water; the pH value of the preservative agent is 7.0-9.5; the DNA in a saliva sample can be effectively preserved at room temperature for a long time; and the defects in the prior art that the preservative time of the DNA acquired from the saliva sample is short and the DNA is required to be preserved at low temperature are effectively overcome. The preservative agent disclosed by the invention is low in cost, simple in preparation method and suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of medical biotechnology, and in particular relates to a saliva DNA preservation agent. Background technique [0002] Compared with the traditional way of drawing blood to collect DNA, saliva DNA sample collection is a method of obtaining DNA that is harmless and painless to the human body, and is not easy to cause discomfort and fear of the collected person. Any discomfort caused by the collector is easily accepted, thus maximizing the sampling range of genetic research, especially suitable for large-scale population investigations in molecular epidemiology. In the prior art, the collection of saliva DNA samples is mainly carried out by using cotton swabs to obtain oral mucosal epithelial cells. The amount of DNA collected by this method is small, and it needs to be stored at low temperature and the storage time is short. Contents of the invention [0003] The purpose of the present invention is to overcome the de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
Inventor 占伟张华能陈丽娟郑小强栾国彦
Owner XIAMEN ZEESAN BIOTECH
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