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Method and device for loading cell fluid stress on deformable curved surface and experimental platform

A technology of loading device and fluid stress, which is applied in the field of biomechanics and can solve problems such as the difficulty of influencing the law of cell morphology and biological characteristics

Inactive Publication Date: 2012-09-19
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is very difficult to observe the influence of fluid stress on cell morphology and biological characteristics in vivo

Method used

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  • Method and device for loading cell fluid stress on deformable curved surface and experimental platform
  • Method and device for loading cell fluid stress on deformable curved surface and experimental platform
  • Method and device for loading cell fluid stress on deformable curved surface and experimental platform

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Embodiment Construction

[0019] Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0020] figure 1 It is a cell stress loading experimental platform, which is composed of a cell fluid stress loading device 1 on a deformable surface, a pressure chamber regulating syringe 2, a peristaltic pump 3, a culture medium 4, a liquid storage tank 5, and a silicone tube 6. In addition, data acquisition and analysis devices such as microscopes, CCD cameras and computers can also be built outside the experimental platform. Among them, the cell fluid stress loading device 1 on the deformable curved surface is the core part of the experimental platform.

[0021] Such as figure 2 As shown, the cell fluid stress loading device 1 on a deformable curved surface includes a first cover sheet 7 , a first sealing gasket 8 , a silicone membrane 9 , a second sealing gasket 10 and a second cover sheet 11 from top to bottom. The first cover sheet 7 ...

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Abstract

The invention discloses a method loading cell fluid stress on a deformable curved surface. A flowing cavity and a pressure cavity are isolated from each other through a transparent elastic membrane; biological cells are inoculated onto the surface of the transparent elastic membrane which faces the flowing cavity; the flowing cavity is full of a cell culture fluid; the transparent elastic membrane is deformed by applying a pressure through the pressure cavity; and the cell culture fluid is driven to circularly flow in the flowing cavity through a peristaltic pump, so that the biological cellsare subjected to the tangential stress and pressure of the fluid. The invention further provides a device for implementing the method. The device comprises a first cover plate, a first sealing gasket, a transparent elastic membrane, a second sealing gasket and a second cover plate from top to bottom, wherein a flowing cavity is formed among the first cover plate, the first sealing gasket and the transparent elastic membrane; and a pressure cavity is formed among the transparent elastic membrane, the second sealing gasket and the second cover plate. The shape of the bottom surface of the flowing cavity where the biological cells are positioned can be changed, and a fluid stress state in which the biological cells are kept is adjusted simultaneously, so that the shape and biological characteristic change rules of biological cells in different stress states are observed and detected.

Description

technical field [0001] The invention relates to the field of biomechanics, in particular to a cell fluid stress loading method and device on a deformable curved surface, which is used for studying the responses of various cells under controllable fluid stress states. Background technique [0002] In medical research, it is found that cells in human tissues (such as vascular endothelial cells, corneal endothelial cells, etc.) are affected by fluid (such as blood, aqueous humor) stress (fluid shear stress and pressure) and undergo morphology (shape, size, cell Density, etc.) and changes in biological characteristics (such as cell endothelial permeability, cytokine expression, DNA synthesis, etc.), so it is of great significance to observe the impact of fluid stress on cells. However, it is very difficult to observe the influence of fluid stress on cell morphology and biological characteristics in vivo. In order to solve this problem, it is necessary to use the method of cultu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00C12M1/00
CPCC12M23/22C12M23/38C12M23/26C12M25/02
Inventor 陈瀚张峰黄渝侃黄鲜荣
Owner HUAZHONG UNIV OF SCI & TECH
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