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Serratia strain and method for disposing methidathion pesticide residues on surfaces of soil and fruits/vegetables

A technology for the degradation of Serratia and pesticide residues, applied in the direction of microorganism-based methods, restoration of contaminated soil, biochemical equipment and methods, etc., can solve problems such as pesticide residue pollution, achieve low production costs, and protect the ecology The effect of environment and ease of use

Inactive Publication Date: 2012-09-19
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of degrading bacterium of methapone insecticide aiming at the practical problems and needs of pesticide residue pollution in current agricultural production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The surface soil of peach orchards in Nongquanyi District, Chengdu, which had been treated with chlorpyrifos for several years, was collected. Weigh 5g of the collected pesticide-contaminated soil sample, pass it through a 20-mesh sieve, and add it to 100ml sterilized basal salt medium containing 50mg / l methapon. The formula of the basal salt medium is (all g / L): NH 4 NO 3 1.0, MgSO 4 ·7H 2 O 0.5, (NH 4 ) 2 SO 4 0.5, KH 2 PO 4 0.5, NaCl 0.5, K 2 HPO 4 1.5, PH: 7.0-7.2, cultured at 37°C with 180r / min shaking. After 7 days, take 5ml of the bacterial liquid and insert it into the same medium for cultivation at 37°C. During the subculture process, the concentration of methionin was gradually increased to 400 mg / l, and the subculture was carried out 6 times, and once in about 7 days. Make a 10-fold gradient of the enriched pesticide-degrading bacteria solution with sterile water (10 - 7 、10 - 8 、10 - 9 ) dilution, respectively take 0.1mL bacterial suspensio...

Embodiment 2

[0026] The slant of Serratia spp. in Example 1 with negative Gram staining was inoculated in 100ml LB medium, and the formula of LB medium (g / l) was as follows: peptone 10, yeast extract 5, NaCl 10, pH 7.0-7.2 , constant temperature shaking culture to the logarithmic phase, to obtain bacterial liquid. Transfer the above-mentioned cultivated bacterial solution into a seed tank that has been autoclaved at 121°C and cooled to about 35°C according to the inoculation amount of 15% by volume. The formula of the seed tank medium is: glucose 5g / L, NH 4 NO 3 10g / L , K 2 HPO 4 2 g / L, MgSO 4 ·7H 2 O 0.5 g / L, Na2 HPO 4 2 g / L, CaCO 3 3g / L, NaCl 1.0 g / L, yeast extract 0.5 g / L, pH: 7.0-7.2; continue to cultivate until the logarithmic growth phase, the ventilation rate of sterile air during the cultivation is 1:1.5, and the stirring speed is 200 revolutions per minute, and the incubation temperature was 35°C. The seed solution that has reached the logarithmic growth phase is inser...

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Abstract

The invention provides a new microorganism strain capable of degrading methidathion pesticide residues, namely (Serratia sp.) SPL-2 with the Genbank accession number of JN400353, and a methidathion pesticide residue degrading bactericide developed using the degrading strain. The degrading bactericide degrades chemical pesticides by use of the microorganism technology, belonging to the technical field of environmental microbial remediation; the production technology is as follows: seedling on an inclined plane->seed liquid in a shake flask->a seed tank->a fermentation tank->a product; the preparation technology is simple, has low production cost and is easy to popularize and use in a large area; when in use, the degrading bactericide is directly sprayed, thereby being very convenient; the bactericide product can quickly and effectively degrade the residual bactericides on the surfaces of soil and fruits / vegetables so as to solve the problem of exceeding residues caused by pesticide spraying in agricultural production; and the green agricultural product without toxicity or pollution can be produced, and the quality and economic value of the agricultural product are effectively improved.

Description

technical field [0001] The invention belongs to the technical field of environmental microbial restoration, and in particular relates to a Serratia strain SPL-2 and a method for treating the residues of thifapon pesticides on the surface of soil and fruits and vegetables. Background technique [0002] Pesticides play an important role in ensuring the increase of food production, and are therefore widely used in agricultural production in countries all over the world. However, the excessive use of pesticides has had serious negative effects. Pesticide residues are difficult to degrade. When people use pesticides to prevent pests and weeds, they also cause pesticide residues in food, vegetables, melons and fruits to exceed the standard, causing serious pollution and causing harm to non-target organisms. In my country, among the many factors that affect the quality and safety of fresh agricultural products such as vegetables and fruits, the most prominent is the excessive pest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/425A62D101/04A62D101/26
Inventor 徐恒李创杨曦
Owner SICHUAN UNIV
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