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In-vitro culture method for tender stem segments of cherry rootstock

A technology of in vitro culture and rootstock, which is applied in horticultural methods, botany equipment and methods, cultivation, etc., can solve the problems of low reproduction rate, easy to carry viruses, and cannot meet the needs of production seedlings, and achieve fast reproduction speed and high reproductive efficiency. high rate effect

Inactive Publication Date: 2012-09-12
上海闵行区苗圃有限公司
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AI Technical Summary

Problems solved by technology

The existing rootstocks for cherry propagation have a low reproductive rate and are prone to viruses, which cannot meet the needs of seedlings for production

Method used

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Examples

Experimental program
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Effect test

Embodiment

[0019] A method for in vitro cultivation of cherry rootstock tender stem segment, the specific steps are:

[0020] Step 1: Select fresh semi-lignified twigs, cut off the leaves, wash twice with detergent, soak and disinfect with 75vol% ethanol solution for 1min on the ultra-clean workbench, and then use 0.1vol% Soak in mercury solution for disinfection for 15 minutes, rinse with sterile water three times, and dry the water with sterile filter paper;

[0021] Step 2: Cut the twigs obtained in the first step into 1 cm long stem segments, each stem segment has 1-2 stem nodes, and inoculate them in the first medium to induce axillary buds to germinate and grow. The temperature of the culture room is 23°C. 12 hours of light per day, light intensity 3000lx, the first medium is obtained by adding zeatin and naphthaleneacetic acid to 1 / 2MS medium, the concentration of zeatin in the first medium is 2mg / L, and the concentration of naphthaleneacetic acid is 0.2mg / L;

[0022] The third...

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PUM

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Abstract

The invention provides an in-vitro culture method for tender stem segments of cherry rootstock. The in-vitro culture method for the tender stem segments of cherry rootstock comprises the following specific steps of: selecting newly-sprouted semi-lignified tender branches, shearing off leaves, cleaning and disinfecting, washing by sterile water, and finally, absorbing water by sterile filter paper; cutting the tender branches into stem segments, inoculating the stem segments in a first culture medium and inducing axillary buds to germinate and grow; cutting off the buds, and inoculating the buds in a second culture medium and inducing clustered buds to grow; cutting the clustered buds into single buds, inoculating the single buds on a third culture medium, and then performing rooting culture to obtain rooted test-tube seedlings; and transplanting the test-tube seedlings in a peat+perlite matrix, placing in a plastic plug and then culturing into plug seedlings, watering enough after transplanting, starting to spraying clean water on leaf surfaces the next day and spraying twice every day, dressing nutrient solution after half month and spraying twice every month, transplanting in a plastic shed after more than three months of culture, and then culturing as rootstock big seedlings. The seedlings of cherry rootstock cultured by the culture method provided by the invention are fast in propagation and high in propagation rate.

Description

technical field [0001] The invention relates to a rapid in vitro culture method for tender stems of cherry rootstocks, which utilizes semi-lignified new branches pumped out in the past year to induce clustered buds after cleaning and disinfection, and then obtains complete test-tube seedlings after culture, and belongs to the technical field of cherry culture methods . Background technique [0002] Cherry (Prunus) is a deciduous tree of the genus Cerasus Mill. in the family Rosaceae. There are more than 100 species of cherry plants, the main cultivated species are Chinese cherry (Prunus pseudoerasus Lindl.), European sweet cherry (Prunus avium L.), European sour cherry (Prunus cerasus L.) and hair cherry (Prunus tomentosa Thub.) kind. Cherry fruit is a high-grade fruit with strong adaptability, rich nutrition, sweet and sour taste, and high economic value. Cherry propagation mainly relies on cuttings and grafting. Due to the constraints of rootstocks, the production and d...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00
Inventor 陆锦明朱天华王水燕汤桂钧蒋建平胡海峰
Owner 上海闵行区苗圃有限公司
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