Detection method for growth state of roots of banana temporarily-planted seedlings

Abstract

The invention discloses a detection method for the growth state of the roots of banana temporarily-planted seedlings, comprising the following steps of: (1) selecting banana temporarily-planted seedlings transplanted into a seedling culture device from a seedbed and then culturing for 7-35 days, cutting off pseudostem parts, and performing cutting treatment in the seedling culture device to obtain the roots of the banana temporarily-planted seedlings; (2) placing the roots of the banana temporarily-planted seedlings in reaction solution containing 2,3,5-triphenyltetrazolium chloride, adjusting the temperature of a reaction to be 37+ / -2 DEG C, and culturing for 2-3 hours in a dark environment to perform colouring reaction; and (3) after the colouring reaction, placing the roots of the banana temporarily-planted seedlings in stopping solution to stop the colouring reaction, taking out the roots of the banana temporarily-planted seedlings, and detecting the colouring condition of each part of the roots of the plants, wherein the higher the ratio of the red roots to the colourless roots in the same area is, the better the growth state of the roots of the banana temporarily-planted seedlings is. By the method, the growth state of the roots of the banana temporarily-planted seedlings can be rapidly and simply detected, a plurality of middle test steps can be reduced, error can be decreased, and operation procedures can be simplified.

Description

technical field [0001] The invention belongs to the technical field of crop physiological cultivation, and in particular relates to a method for detecting the growth state of roots of banana pseudo-plants. Background technique [0002] Banana (Musa spp.) belongs to the perennial herbaceous plant of Musaceae (Musacease) and is a perennial evergreen herb in the tropics and subtropics. Banana has a huge underground root system, and the health of the root system has a great influence on the ability to absorb nutrients and water. extremely important role. Ripe bananas can usually be pumped from the surface of the central column of the bulb in groups of four, with a root system diameter of 5-8 mm, in numbers of 200-400, sometimes as many as 700. Distributed in the soil layer above 30cm, forming a horizontal root system, the length of which can reach more than 3m. In related reports, there are few studies on the root system of Musa plants in the later stage of growth. The fundamen...

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Problems solved by technology

Distributed in the soil layer above 30cm, forming a horizontal root system, the length of which can reach more than 3m. In related reports, there are few studies on the root system of Musa plants in the later stage of growth. The fundamental reason is that the excavation of the entire root system is cumbersome and time-consuming. The root system is vulnerable to damage during digging and cannot reflect the original

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