TTC (2,3,5-triphenyltetrazolium chloride) chromogenic culture medium for drug sensitivity tests, drug sensitivity kit, preparation method of drug sensitivity kit and drug sensitivity test method

Abstract

The invention provides a TTC (2,3,5-triphenyltetrazolium chloride) chromogenic culture medium for drug sensitivity tests. The TTC chromogenic culture medium comprises BHI (brain heart infusion) broth, acid-hydrolyzed protein, soluble starch and TTC. The invention further provides a drug sensitivity kit. The drug sensitivity kit comprises a kit part and a drug-sensitive plate part, wherein the kit part comprises the TTC chromogenic culture medium. The invention further provides a preparation method of the drug sensitivity kit. The preparation method comprises preparation of the drug-sensitive plate part and preparation of the TTC chromogenic culture medium, and the drug-sensitive plate part is provided by the invention. The invention further provides a drug sensitivity test method. The drug sensitivity test method comprises steps of diluting a bacterial suspension with the culture medium, adding the diluted bacterial suspension to drug-sensitive holes and performing culture; the culture medium is the TTC chromogenic culture medium. With the adoption of the TTC chromogenic culture medium, the drug-sensitivity kit, the drug sensitivity test method and the drug sensitivity kit prepared with the preparation method of the drug sensitivity kit, drug sensitive test results can be directly and easily observed, deviation cannot be caused easily, drug sensitive report time is greatly shortened to only 4-6 h.

Description

technical field [0001] The invention belongs to the technical field of microbial detection. Specifically, the invention relates to a TTC chromogenic medium for drug sensitivity test, a drug sensitivity test kit, a preparation method thereof, and a drug sensitivity test method. Background technique [0002] Different bacteria will have different reactions to different antibiotics. If they are sensitive to a certain antibiotic, the antibiotic can inhibit the growth and reproduction of bacteria, and then provide a reference for the selection of antibiotics for treatment. We call this process drug sensitivity test . [0003] Traditional drug sensitivity laboratories judge whether bacteria are sensitive to a certain antibiotic by the principle of turbidimetry, and cannot visually observe the results of drug sensitivity tests. Contents of the invention [0004] In order to overcome the above technical problems, the present invention provides a TTC chromogenic culture medium for...

AI Technical Summary

Problems solved by technology

[0003] The traditional drug sensitivity laboratory judges whether the bacteria are sensitive to a certain antibiotic through the principle of turbidimetry, and cannot directly observe the results of the drug sensitivity test