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Preparation method of glutathione-enriched yeast

A technology of glutathione and yeast, which is applied in the field of preparation of glutathione-rich yeast, can solve the problem of low glutathione content, achieve the effect of increasing added value, good improvement effect, and small loss of activity

Inactive Publication Date: 2012-09-05
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a preparation method of glutathione-rich yeast, which can effectively solve the problem of low glutathione content in microbial glutathione dry yeast, and broaden the use of glutathione-rich dry yeast in the baking field range of applications and increase product added value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment one: Fermentation culture prepares glutathione-rich yeast in shake flask

[0021] (1) Strains:

[0022] Saccharomyces cerevisiae( Saccharomyces cerevisiae ) CICC1532, preserved in the 209 Laboratory of Bioengineering, Hubei University of Technology.

[0023] (2) Medium:

[0024] Each 1L of the slant medium contains: 20g of glucose, 20g of peptone, 5g of yeast extract, 20g of agar, and the pH of the slant medium is 5.8-6.2;

[0025] Each 1L of the seed medium contains: 20g of glucose, 20g of peptone, 5g of yeast extract, and the pH of the slant medium is 5.8-6.2;

[0026] The fermentation medium per 1 L of the shake flask level contains: 25.95 g of glucose, 11.47 g of yeast powder, 10.16 g of ammonium sulfate, 2 g of potassium dihydrogen phosphate, and 0.2 g of magnesium sulfate, and the pH of the fermentation medium is 5.5-6.0;

[0027] (3) Expanded cultivation of seeds:

[0028] After the slant seeds are activated for 12-16 hours, they are cultured ...

Embodiment 2 0

[0046] Embodiment two 30L fermentor adopts fed-batch fermentation culture to prepare glutathione-rich yeast

[0047] (1) strain

[0048] Saccharomyces cerevisiae( Saccharomyces cerevisiae ) CICC1532, preserved in the 209 Laboratory of Bioengineering, Hubei University of Technology.

[0049] (2) Medium

[0050] Each 1L of the slant medium contains: 20g of glucose, 20g of peptone, 5g of yeast extract, 20g of agar, and the pH of the slant medium is 5.8-6.2;

[0051] Each 1L of the seed medium contains: 20g of glucose, 20g of peptone, 5g of yeast extract, and the pH of the slant medium is 5.8-6.2;

[0052] The fermentation medium on each 1 L of the shake flask contains: 25.95 g of glucose, 11.47 g of yeast powder, 10.16 g of ammonium sulfate, 2 g of potassium dihydrogen phosphate, and 0.2 g of magnesium sulfate, and the pH of the fermentation medium is 5.5-6.0;

[0053] The fermentation medium on the level of each 1L of the fermenter contains: 9L of water, 100g of yeast powder, ...

Embodiment 3

[0073] Embodiment three: adopt fed-batch fermentation culture on 60 cubic fermentation tanks

[0074] (1) strain

[0075] Saccharomyces cerevisiae( Saccharomyces cerevisiae ) CICC1532, preserved in the 209 Laboratory of Bioengineering, Hubei University of Technology.

[0076] (2) Medium

[0077] Each 1L of the slant medium contains: 20g of glucose, 20g of peptone, 5g of yeast extract, 20g of agar, and the pH of the slant medium is 5.8-6.2;

[0078] Each 1L of the primary seed medium contains: 20g of glucose, 20g of peptone, 5g of yeast extract, and the pH of the slant medium is 5.8-6.2;

[0079] Each 1L of the secondary seed medium contains: 20g of glucose, 20g of peptone, 5g of yeast extract, and the pH of the slant medium is 5.8-6.2;

[0080] The tertiary seed medium per 1L contains: 20g of glucose, 20g of peptone, 5g of yeast extract, and the pH of the slant medium is 5.8-6.2;

[0081] The fermentation medium on the level of each 1L of the fermenter contains: 16 cubi...

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Abstract

The invention relates to a preparation method of a glutathione-enriched yeast. Culture conditions, feeding strategy and related techniques are optimized to obtain a glutathione-enriched efficient dough yeast product. The method provided by the invention can effectively solve the problems of low glutathione content and poor improvement effect on the dough in the common dried yeast, and the problems of high cost, high oxidation tendency and the like when independently adding glutathione to improve the dough, thereby enhancing the added value of the product.

Description

technical field [0001] The invention relates to a preparation method of glutathione-rich yeast, in particular to a method for preparing glutathione-rich yeast by using agricultural and sideline resources. technical background [0002] Glutathione (GSH) is an antioxidant peptide, a biologically active tripeptide compound containing γ-glutamyl and sulfhydryl groups condensed from L-glutamic acid, L-cysteine ​​and glycine , has important physiological functions such as scavenging free radicals, detoxifying, inhibiting aging, preventing diabetes and cancer, inhibiting HIV, and improving human immunity, and has broad market prospects in the fields of medicine, food, and cosmetics. [0003] The production methods of glutathione mainly include extraction method, chemical synthesis method, enzymatic method and fermentation method. Among them, the fermentation method is the most potential method. However, the current domestic glutathione fermentation production has the following pr...

Claims

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Application Information

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IPC IPC(8): C12N1/18C12R1/865
Inventor 蔡俊王常高
Owner HUBEI UNIV OF TECH
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