Combined preparation process of sophora flavescens total flavone and sophora flavescens total alkaloid

Abstract

The invention provides a combined preparation process of sophora flavescens total flavone and sophora flavescens total alkaloid. The combined preparation process comprises the following steps: crushing raw sophora flavescens; extracting by using low-carbon alcohol; concentrating an extraction solution into extract; dissolving the extract by using acid water to obtain an acid water layer and a solid; washing the solid with water till the solid is neutral; dissolving the solid with a proper amount of ethanol; separating a solution by using polyamide resin; eluting with acid alcohol; collecting eluate; concentrating and drying the eluate to obtain high-purity sophora flavescens total flavone; taking the acid water layer, blending with alkali and precipitating; filtering out precipitates, and dissolving the precipitates with acetone; adding ammonia water into an acetone solution, and separating out and crystallizing; filtering out crystals, and refluxing and dissolving the crystals with 80-95% ethanol to obtain the sophora flavescens total alkaloid. The combined preparation process is easy to operate; the utilization ratio of the raw material is high; the sophora flavescens total flavone and the sophora flavescens total alkaloid with relatively high purity are prepared; and the combined preparation process is easy to expand.

Description

Technical field: [0001] The invention belongs to the technical field of extracting effective components of natural plants, and in particular relates to a method for preparing total flavonoids and total alkaloids of Sophora flavescens from Sophora flavescens as raw materials. Background technique: [0002] Sophora flavescens is the dried root of Sophora flavescens Ait., a perennial deciduous subshrub of the leguminous family Sophora genus. It has the effects of clearing heat and dampness, killing insects and diuresis, and can be used as bitter stomach invigorating agent, diuretic, anti-inflammatory drug, antidiarrheal drug and anthelmintic. [0003] The main functional components of Sophora flavescens are flavonoids and alkaloids. There are few existing techniques for simultaneously extracting flavescens flavonoids and total alkaloids of Sophora flavescens. Extract method", the method disclosed in this patent is to obtain the extract through the steps of extraction, filtrati...

AI Technical Summary

Problems solved by technology

[0003] The main functional components of Sophora flavescens are flavonoids and alkaloids. There are few existing techniques for simultaneously extracting flavescens and total alkaloids of Sophora flavescens. Extract method", the method disclosed in this patent is to obtain the extract through the steps of extraction, filtration, concentration, drying and other separation methods combined with macroporous resin, but this method does not involve further separation of Sophora flavescens alkaloids and flavonoids, Only the crude extracts of Sophora flavescens and Sophora flavescens should be obtained, and the purity is not high; another example is the patent "A Method for Producing Sophora Sophora flavonoids and flavonoid salts", the method used in this patent is to extract with acid water first alkaloids until complete, and then use alcohol to extract flavonoids from Sophora flavescens, and the alcohol extract is processed to obtain flavonoids and flavonoid salts with higher purity. Mention of alkaloid purification steps yields only flavonoids and flavonoid salts