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Method for improving entomopathogenic nematodes infection capability

An entomopathogenic nematode and capacity technology, applied in the field of efficient entomopathogenic nematode live reproduction, can solve problems such as low infection level, and achieve the effects of increasing yield, increasing infection level, and enhancing virulence

Inactive Publication Date: 2012-08-22
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to solve the problem of the low level of infection of entomopathogenic nematodes to Tenebrio molitor, and to provide a method for the living reproduction of entomopathogenic nematodes with high efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0006] Embodiment 1: This embodiment is a method for improving the infectivity of entomopathogenic nematodes, which is specifically completed in the following steps:

[0007] First, dry-heat the Tenebrio molitor larvae at a temperature of 41°C-43°C for 20-30min, and then put the dry-heated Tenebrio molitor larvae into the entomopathogenic nematode suspension for 1 second to complete the treatment of entomopathogenic nematodes on yellow powder. Infestation by worm larvae.

[0008] This embodiment uses physical methods (dry heat treatment at a constant temperature of 41°C to 43°C) to treat host insects, break the defense system of Tenebrio molitor to entomopathogenic nematodes, improve the infection level of entomopathogenic nematodes, and make Tenebrio molitor infestation rate from 55%-57% increased to 95%-98%, thereby increasing the yield of entomopathogenic nematodes, and the toxicity of entomopathogenic nematodes to hosts is also slightly enhanced.

specific Embodiment approach 2

[0009] Specific embodiment two: the difference between this embodiment and specific embodiment one is: first, the Tenebrio molitor larvae are subjected to dry heat treatment for 25 minutes at a temperature of 42°C, and then the Tenebrio molitor larvae after the dry heat are put into the entomopathogen of the infecting stage Dipping the nematode suspension for 1 second completes the impregnation of Tenebrio molitor by entomopathogenic nematodes.

specific Embodiment approach 3

[0010] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: the entomopathogenic nematode is Heterorhabditis bacteriophora.

[0011] Adopt following test to verify effect of the present invention:

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Abstract

The invention provides a method for improving entomopathogenic nematodes infection capability and relates to an entomopathogenic nematodes infection method. The invention aims at solving the problem of low infection level of entomopathogenic nematodes on mealworm. The method is concretely completed according to the following steps that: firstly, a mealworm constant temperature culture box is subjected to dry heat treatment, then, the mealworm subjected to the dry heat treatment is placed into injection period entomopathogenic nematodes suspension to be soaked, and the infection of the entomopathogenic nematodes on the mealworm is completed. The method has the advantage that the injection rate of the mealworm is improved from 55 to 57 percent to 95 to 98 percent. The method is mainly used for the injection of the entomopathogenic nematodes on the mealworm.

Description

technical field [0001] The invention relates to a high-efficiency entomopathogenic nematode live propagation method. Background technique [0002] Entomopathogenic Nematode (EPN) is an important natural enemy of pests, has a wide range of host insects, and is an important means of biological control of pests. The low-cost and high-efficiency mass reproduction of entomopathogenic nematodes is the prerequisite for its popularization and application. Its small-scale propagation technology is mainly the in vivo culture method. In the past, most of the host insects that are sensitive to pathogenic nematodes - Galleria mellonella Linnaeus are used, and the cost is relatively high. Tenebrio molitor is a commonly used cheap host that can replace the greater wax moth. The cost of using Tenebrio molitor as a nematode host for live reproduction is only 25% of that of greater wax moth. Therefore, in the current live reproduction technology, it is a key step for the pathogenic nematode...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 李春杰司兆胜韩新华
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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