Application of paclitaxel in preparation of medicament for treatment of EGFR-TKI acquired resistance
An EGFR-TKI, acquired technology, applied in the field of application of paclitaxel in the preparation of drugs for the treatment of EGFR-TKI acquired drug resistance
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0018] 1. Cell culture: H1975 cells are EGFR L858R-T790M mutant and are resistant to EGFR-TKI; H820 cells have MET amplification and are resistant to EGFR-TKI; A549 cells express wild-type EGFR gene and are resistant to EGFR-TKI; PC- 9 lines of EGFR-TKI highly sensitive cells, the above-mentioned H1975, H820, and PC-9 cells were purchased from ATCC (USA), and A549 cells were stored in our laboratory. Cell culture uses RPMI-1640 medium (containing 10% FBS, glutamine and penicillin) to subculture cell lines H1975, H820, A549, PC-9. Cell culture was performed in an incubator containing 5% CO2 at 37°C, and the culture medium was replaced every 1 to 2 days. The four types of cells are adherent cells. When the cells grow to a confluency of about 90%, they are digested with trypsin, and repeatedly pipetted to leave the bottom wall of the culture flask, and then collected by centrifugation. Half of the collected cells are used for RNA extraction and half for protein extraction.
[001...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com