Molecular identification method for cotton restoring line containing sterile cytoplasm of gossypium harknessii

Abstract

The invention belongs to the field of biological technique, relates to a molecular identification method for the purity of a cotton restoring line, and particularly provides a primer of a SSR (Simple Sequence Repeat) marker for identifying the cotton restoring line containing the sterile cytoplasm of gossypium harknessii. The primer is as shown in nucleotide sequences such as SEQ ID No.1 and 2 and / or SEQ ID No. 3 and 4. The SSR marker is adopted for carrying out polymorphism detection of molecular level on the cotton restoring line material containing the sterile cytoplasm of the gossypium harknessii. The molecular identification method is fast in identification speed, high in accuracy and simple in operation and is very suitable for the indoor identification of the cotton restoring line and the assisted-selective breeding of molecular markers.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular identification method for cotton restorer line purity identification and molecular marker-assisted breeding. Background technique [0002] The research and utilization of heterosis has a significant effect on increasing the yield, quality and resistance of cotton. At present, most cotton farmers in my country's seedling and transplanting areas have planted hybrid cotton; and the cotton areas in the Yellow River Basin, and even direct cotton areas such as Xinjiang are also vigorously developing hybrid cotton. However, the production mode of cotton hybrid seeds is mainly artificial detasseling assisted pollination hybrid seed production method (accounting for more than 90%). With the increase of labor costs in major cotton production areas and their surrounding areas year by year, the cost of seed production has become higher and higher, which greatly limits th...

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problems in the prior art that the field identification and detection method of the cotton restorer line selection containing Harknessy cotton cytoplasmic male sterile cytoplasm takes a long time, consumes a lot, and has poor accuracy. Molecular identification method of Hackney West cotton sterile cytoplasmic cotton restorer line, so as to speed up the process of restorer line selection and improvement, and at the same time ensure the purity of restorer line seeds

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