Method for detecting insecticide resistance of schistosoma japonicum miracidia to praziquantel in vitro

An in vitro detection and praziquantel technology, applied in biological testing, material inspection products, etc., can solve the problems of drug resistance, long time, missed detection and treatment, etc., and achieve the effect of solving on-site detection and monitoring, and solving technical problems

Inactive Publication Date: 2012-07-25
JIANGSU INST OF PARASITIC DISEASES
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  • Description
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Problems solved by technology

Long-term and large-scale repeated application of the same insecticide will often lead to drug resistance, so it is necessary to establish on-site monitoring and detection technology for praziquantel resistance
The current main method of detectin

Method used

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  • Method for detecting insecticide resistance of schistosoma japonicum miracidia to praziquantel in vitro
  • Method for detecting insecticide resistance of schistosoma japonicum miracidia to praziquantel in vitro

Examples

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Effect test

Embodiment 1

[0009] Example 1: Sensitivity determination of Schistosoma japonicum praziquantel-sensitive and resistant strain miracidium to praziquantel

[0010] Two petri dishes with a diameter of 5 cm were taken, and 1 mL of water containing 40-120 freshly hatched praziquantel-resistant and praziquantel-resistant strains of Schistosoma japonicum were respectively transferred into 1 mL of water, and then 1 mL of praziquantel liquid was added quickly, Make the concentration of the drug solution in the petri dish 5×10 -7 mol / L, Lugol's iodine solution was added dropwise after 1 min, the morphological changes were observed under a dissecting microscope, and the deformation rates of miracidia of sensitive and resistant strains were calculated. The experiment was repeated 6 times. The results are shown in Table 1. Taking the water containing blood-sucking miracidium without adding praziquantel as a control, the miracidium was oval or oval without deformation.

[0011] Table 1 Effects of pr...

Embodiment 2

[0013] Example 2: Sensitivity determination of Schistosoma japonicum praziquantel-sensitive and resistant strain miracidium to praziquantel

[0014] Two petri dishes with a diameter of 5 cm were taken, and 1 mL of water containing 40-120 freshly hatched praziquantel-resistant and praziquantel-resistant strains of Schistosoma japonicum were respectively transferred into 1 mL of water, and then 1 mL of praziquantel liquid was added quickly, The concentration of the liquid medicine in the petri dish was respectively 5×10 -7 mol / L, after 3 min, Lugol's iodine solution was added dropwise, the morphological changes were observed under a dissecting microscope, and the deformation rates of miracidia of sensitive strains and resistant strains were calculated. The experiment was repeated 6 times. The results are shown in Table 2.

[0015] Table 2 Effects of praziquantel on the morphological changes of miracidia of praziquantel-sensitive and resistant strains of Schistosoma japonicum ...

Embodiment 3

[0017] Example 3: Sensitivity determination of Schistosoma japonicum praziquantel-sensitive and resistant strain miracidium to praziquantel

[0018] Two petri dishes with a diameter of 5 cm were taken, and 1 mL of water containing 40-120 freshly hatched praziquantel-resistant and praziquantel-resistant strains of Schistosoma japonicum were respectively transferred into 1 mL of water, and then 1 mL of praziquantel liquid was added quickly, The concentration of the liquid medicine in the petri dish was respectively 5×10 -7 mol / L, after 5 min, Lugol's iodine solution was added dropwise, the morphological changes were observed under a dissecting microscope, and the deformation rates of miracidia of sensitive and resistant strains were calculated. The experiment was repeated 6 times. The results are shown in Table 3.

[0019] Table 3 Effects of praziquantel on the morphological changes of miracidia of praziquantel-sensitive and resistant strains of Schistosoma japonicum

[0020...

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Abstract

The invention relates to a method for detecting insecticide resistance of schistosoma japonicum miracidia to praziquantel in vitro, belonging to the technical field of antiparasitic drugs. The method comprises the following steps of: taking a culture dish with a diameter of 5cm; first immigrating water of 1mL containing 40-120 new hatched schistosoma japonicum miracidia, then quickly adding praziquantel liquid medicine of 1mL, and enabling the concentration of liquid medicine in the culture dish to respectively be 5*10-7mol/L and 10-7mol/L; dropwise adding Lugol's iodine solution after 1min, 3min and 5 min; observing the morphologic changes of the miracidia under an anatomical lens, and calculating the deformation rate of the miracidia; and repeating the experiment for six times, and detecting the insecticide resistance of the schistosoma japonicum miracidia to praziquantel by observing the morphologic changes of the miracidia. The method is fast, simple, convenient, sensitive and reliable and is suitable for fast detecting and monitoring generation of the insecticide resistance of the schistosoma japonicum miracidia to praziquantel. According to the method, the technical problem of on-site detection and monitor of the insecticide resistance of the schistosoma japonicum miracidia to praziquantel can be realized.

Description

technical field [0001] The invention discloses a method for in vitro detection of resistance of Schistosoma japonicum miracidium to praziquantel, which belongs to the technical field of antiparasitic drugs. Background technique [0002] Schistosomiasis japonicum is a major infectious disease that endangers human health and affects social and economic development. Praziquantel is the only commercially available drug currently used to treat schistosomiasis japonicum. Long-term and large-scale repeated application of the same insecticide will often lead to drug resistance, so it is necessary to establish on-site monitoring and detection technology for praziquantel resistance. The current main method of detecting praziquantel resistance is to treat patients on-site in the epidemic area and evaluate the results of chemotherapy. This method takes a long time, the sequence of patients is low, and there is a phenomenon of missed detection and treatment. We have established a metho...

Claims

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Application Information

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IPC IPC(8): G01N33/50
Inventor 梁幼生汪伟李洪军戴建荣曲国立邢云天
Owner JIANGSU INST OF PARASITIC DISEASES
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