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Cultivation method for halophilic bacteria capable of efficiently degrading high-salinity wastewater phenol and application method of halophilic bacteria

A technology of application method and cultivation method, which is applied in the field of sewage treatment, can solve the problems of secondary pollution, uneconomical, high operating costs of ozone oxidation, etc., and achieve the effect of strong environmental adaptability and wide source of bacteria

Inactive Publication Date: 2012-07-25
CHANGZHOU YAHUAN ENVIRONMENTAL PROTECTION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Photocatalytic oxidation, wet catalytic oxidation, and ozone oxidation are all costly and uneconomical, and these methods are likely to cause secondary pollution in treating phenolic wastewater

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0019] In the beef extract peptone medium, take 1g of tryptone, 0.2g of beef extract, 8.8g of NaCl, 1g of Epsom salt, 1g of iron fumarate, 1g of M-aspartic acid, 1g of vegetable oil, 1g of agar, and 85g of salted brine in the beef extract peptone medium by mass. .

[0020] In the phenol culture solution, 10 g of actinomycin, 20 g of NaCl, 20 g of iron fumarate, 10 g of bromothymol blue, 240 g of phenol, and 800 g of pickled brine were taken by mass in the phenol culture solution.

[0021] The cultivation method of high salinity halophilic bacteria is as follows:

[0022] The Great Salt Lake water was taken as the bacterial source, cultured in beef extract peptone medium with a salt content of 15%, and the obtained mixture was put into a centrifuge tube by the scraper method, and centrifuged at 10000r / min for 10min. Take the supernatant as the bacterial source of moderately halophilic bacteria that reduce phenol, and store it in cold storage; then inoculate the halophilic bact...

example 2

[0024] In the beef extract peptone medium, take 3g of tryptone, 0.5g of beef extract, 6.5g of NaCl, 3g of Epsom salt, 3g of iron fumarate, 3g of M-aspartic acid, 3g of vegetable oil, 3g of agar, and 75g of pickling brine in terms of mass in the beef extract peptone medium .

[0025] In the phenol culture solution, by mass, 20 g of actinomycin, 50 g of NaCl, 50 g of iron fumarate, 30 g of bromothymol blue, 250 g of phenol, and 600 g of pickled brine.

[0026] The cultivation method of high salinity halophilic bacteria is as follows:

[0027] The Great Salt Lake water was taken as the bacterial source, cultured in beef extract peptone medium with a salt content of 18%, and the obtained mixture was put into a centrifuge tube by the scraper method, and centrifuged at 10000r / min for 10min. Take the supernatant as the bacterial source of moderately halophilic bacteria that reduce phenol, and store it in cold storage; then inoculate the halophilic bacteria source stored in cold sto...

example 3

[0029] In the beef extract peptone medium, take 2g of tryptone, 0.4g of beef extract, 7.6g of NaCl, 2g of Epsom salt, 2g of iron fumarate, 2g of M-aspartic acid, 2g of vegetable oil, 2g of agar, and 80g of salted brine in the beef extract peptone medium. .

[0030] In the phenol culture solution, by mass, 15 g of actinomycin, 25 g of NaCl, 25 g of iron fumarate, 20 g of bromothymol blue, 215 g of phenol, and 700 g of pickled brine.

[0031] The cultivation method of high salinity halophilic bacteria is as follows:

[0032] The Great Salt Lake water was taken as the bacterial source, cultured in the beef extract peptone medium with a salt content of 17%, the obtained mixture was put into a centrifuge tube by the scraper method, and centrifuged at 10000r / min for 10min. Take the supernatant as the bacterial source of moderately halophilic bacteria that reduce phenol, and store it in cold storage; then inoculate the halophilic bacteria source stored in cold storage into the ...

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PUM

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Abstract

The invention discloses a cultivation method for halophilic bacteria capable of efficiently degrading high-salinity wastewater phenol and an application method of the halophilic bacteria and belongs to the technical field of sewage treatment. The halophilic bacteria in water of a great salt lake are selected by using a beef extract peptone culture medium of which the salinity is 15 to 18 percent, are cultivated by using a phenol liquid culture medium, and are acclimatized through a sequencing batch reactor (SBR), and then the halophilic bacteria are prepared. The halophilic bacteria are low in cost and high in environment adaptability. The problem that medium halophilic bacteria are time-consuming in removing the high-salinity wastewater phenol is solved. The halophilic bacteria have obvious economic benefit, social benefit and wide application prospect.

Description

technical field [0001] The invention relates to the cultivation and application method of halophilic bacteria degrading high-salinity wastewater phenol, belonging to the field of sewage treatment. Background technique [0002] In the process of industrial production, a large amount of high-salinity phenolic wastewater will be produced, such as pesticides and herbicides, and a large amount of inorganic salts are used in the production process; dyes are used in refining and bleaching processes, oil and natural gas The mining process is medium. In the production process, although the pollutants in the wastewater are required to be controlled at the source, due to some technical reasons, the organic matter in the high-salinity wastewater is difficult to treat. [0003] Phenolic substances present in high-salt wastewater not only pose a serious threat to human health, but also bring certain toxicity to aquatic products and aquatic organisms. When the phenol content in the wat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/34
Inventor 雷春生雷思宇包铠
Owner CHANGZHOU YAHUAN ENVIRONMENTAL PROTECTION TECH
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