Cultivation method for halophilic bacteria capable of efficiently degrading high-salinity wastewater phenol and application method of halophilic bacteria
A technology of application method and cultivation method, which is applied in the field of sewage treatment, can solve the problems of secondary pollution, uneconomical, high operating costs of ozone oxidation, etc., and achieve the effect of strong environmental adaptability and wide source of bacteria
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example 1
[0019] In the beef extract peptone medium, take 1g of tryptone, 0.2g of beef extract, 8.8g of NaCl, 1g of Epsom salt, 1g of iron fumarate, 1g of M-aspartic acid, 1g of vegetable oil, 1g of agar, and 85g of salted brine in the beef extract peptone medium by mass. .
[0020] In the phenol culture solution, 10 g of actinomycin, 20 g of NaCl, 20 g of iron fumarate, 10 g of bromothymol blue, 240 g of phenol, and 800 g of pickled brine were taken by mass in the phenol culture solution.
[0021] The cultivation method of high salinity halophilic bacteria is as follows:
[0022] The Great Salt Lake water was taken as the bacterial source, cultured in beef extract peptone medium with a salt content of 15%, and the obtained mixture was put into a centrifuge tube by the scraper method, and centrifuged at 10000r / min for 10min. Take the supernatant as the bacterial source of moderately halophilic bacteria that reduce phenol, and store it in cold storage; then inoculate the halophilic bact...
example 2
[0024] In the beef extract peptone medium, take 3g of tryptone, 0.5g of beef extract, 6.5g of NaCl, 3g of Epsom salt, 3g of iron fumarate, 3g of M-aspartic acid, 3g of vegetable oil, 3g of agar, and 75g of pickling brine in terms of mass in the beef extract peptone medium .
[0025] In the phenol culture solution, by mass, 20 g of actinomycin, 50 g of NaCl, 50 g of iron fumarate, 30 g of bromothymol blue, 250 g of phenol, and 600 g of pickled brine.
[0026] The cultivation method of high salinity halophilic bacteria is as follows:
[0027] The Great Salt Lake water was taken as the bacterial source, cultured in beef extract peptone medium with a salt content of 18%, and the obtained mixture was put into a centrifuge tube by the scraper method, and centrifuged at 10000r / min for 10min. Take the supernatant as the bacterial source of moderately halophilic bacteria that reduce phenol, and store it in cold storage; then inoculate the halophilic bacteria source stored in cold sto...
example 3
[0029] In the beef extract peptone medium, take 2g of tryptone, 0.4g of beef extract, 7.6g of NaCl, 2g of Epsom salt, 2g of iron fumarate, 2g of M-aspartic acid, 2g of vegetable oil, 2g of agar, and 80g of salted brine in the beef extract peptone medium. .
[0030] In the phenol culture solution, by mass, 15 g of actinomycin, 25 g of NaCl, 25 g of iron fumarate, 20 g of bromothymol blue, 215 g of phenol, and 700 g of pickled brine.
[0031] The cultivation method of high salinity halophilic bacteria is as follows:
[0032] The Great Salt Lake water was taken as the bacterial source, cultured in the beef extract peptone medium with a salt content of 17%, the obtained mixture was put into a centrifuge tube by the scraper method, and centrifuged at 10000r / min for 10min. Take the supernatant as the bacterial source of moderately halophilic bacteria that reduce phenol, and store it in cold storage; then inoculate the halophilic bacteria source stored in cold storage into the ...
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