Preparation method of fungus fermentation product and application thereof in prevention and treatment of rice diseases

A technology of fermentation products and fungi, which is applied in the field of preparation of fungal fermentation products, can solve problems such as unresolved industrial production technology and no formal commercial products, and achieve the effects of easy industrial production, good control effect, and simple preparation method

Inactive Publication Date: 2012-07-25
FUJIAN NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The research on fungal insecticides has a long history in our country, and a lot of work has been done on the safety of the preparations, the control...

Method used

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  • Preparation method of fungus fermentation product and application thereof in prevention and treatment of rice diseases
  • Preparation method of fungus fermentation product and application thereof in prevention and treatment of rice diseases
  • Preparation method of fungus fermentation product and application thereof in prevention and treatment of rice diseases

Examples

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preparation example Construction

[0025] The preparation method of fungal fermentation product provided by the invention, its steps are:

[0026] 1) Liquid culture and fermentation product treatment: the fungus Agrocybe sp. YB2005 (preserved in China Center for Type Culture Collection (CCTCC) on March 12, 2012, address: Wuhan University, deposit number CCTCC NO: 2012076) slant strains were first inoculated in a Erlenmeyer flask containing 150mL potato liquid medium Medium-medium activation, the activation conditions are rotation speed 230 r / min, culture temperature 28°C, culture time 7 days, and then carry out large-scale liquid submerged fermentation. The medium formula used is: 200 g of potatoes, 20 g of glucose, and 5 g of peptone per liter of water , natural pH, 0.1Mpa, sterilized at 121°C for 30 minutes, and cultured in a constant temperature shaker at 25-28°C, 180-230 r / min. After 7-12 days of fermentation, the mycelium is separated from the fermentation broth by centrifugation. The fermented liquid ...

Embodiment 1

[0034] Using potato glucose medium (containing 200g of potato, 20g of glucose, 5g of peptone per liter of water, natural pH, 0.1Mpa, sterilized at 121°C for 30min), culture 10 L of activated strain YB2005 in a shaker at 28°C Cultured for 9 d. After the cells were filtered, the fermentation broth was concentrated to 0.5 L, and the fermentation broth was extracted with ethyl acetate. The ethyl acetate part was dehydrated with anhydrous sodium sulfate and then concentrated, then dissolved in methanol and filtered to obtain methanol-soluble crude extract (1.2 g, brown extract).

[0035] Dissolve 1.2 g of the crude methanol extract in the previous step with an appropriate amount of methanol, go through gel (140 g ) column chromatography, and elute with methanol at a flow rate of about 10-15 s / drop, collect 5 mL in each test tube, similar Fractions were combined to obtain Fraction Fr.4 (111 mg) respectively.

[0036] Take 50 mg from Fr.4 (111 mg) in the previous step and dissolve ...

Embodiment 2

[0040] Similar to Example 1, the difference is that the compound structure is identified by NMR (nuclear magnetic resonance) analysis, and the compound structure can be determined according to the compound NMR and MS data.

[0041] from compound 13 C-NMR data show that there are 8 carbons in its structure, and according to the chemical shift value, it is speculated that it is 6 alkyne carbons, 1 oxymethylene and 1 amido carbon (d 153.9s). Using MeOD as the tritiated reagent 1 H-NMR shows that there are two isocarbon protons with the same chemical shift value in its structure, and DMSO is used as the tritiated reagent 1 H-NMR also shows that there are two isocarbon protons with the same chemical shift value in its structure, and three active hydrogens (d 8.42(s,1H), d 7.97(s,1H) and d 5.63(t,6.1,1H) )). The structure of the compound was determined based on the HMBC long-range correlations of H-C(8) with C(7), C(6), C(5) and C(4), which was also supported by the HMBC long-ran...

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Abstract

The invention relates to a preparation method of a fungus fermentation product and application of the fungus fermentation product in prevention and treatment of rice diseases, which belong to the field of biological prevention and treatment. The preparation method of the fungus fermentation product comprises liquid culture of fungus of Agrocybe sp. YB2005, treatment of the fermentation product, and separation of the fermentation product. The fungus fermentation product prepared by the method disclosed by the invention has better effects of prevention and treatment of rice bacterial leaf streak, rice sheath blight, and rice blast, and can be prepared by fermentation of a strain of the Agrocybe sp. YB2005, wherein culture medium raw materials have extensive sources. The preparation method of the fungus fermentation product is simple and easy for realization of industrial production.

Description

technical field [0001] The invention relates to a preparation method of a fungal fermentation product, which can be used for the prevention and treatment of rice diseases and belongs to the field of biological control. Background technique [0002] Macrofungi not only have a wide variety, but also are biological groups with a high "creation coefficient". Natural products found in macrofungi are diverse in structure and possess various biological activities such as antibacterial, promoting nerve growth factor synthesis, enzyme inhibitor, antifungal, cytotoxic, receptor antagonist, and antioxidant. [0003] Basidiomycetes are closely related to humans. After discovering that some species are effective in anti-tumor and preventing coronary heart disease, they have been paid attention to by the medical community and have aroused great interest. The physiologically active substances produced by basidiomycetes include polysaccharides, polypeptides and proteins, antibiotics, vita...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P1/00A01P3/00C12R1/645
Inventor 郑永标沈月毛黄建忠张建福朱永生刘艳如谢华安
Owner FUJIAN NORMAL UNIV
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