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Preparation method of polysaccharide molecule fragment composite coating

A composite coating and fragment technology, applied in the direction of coatings, catheters, packaging items, etc., can solve the problems of poor anticoagulant activity and poor biocompatibility, and achieve the effects of low cost, improved degradation performance, and low natural toxicity

Inactive Publication Date: 2013-11-06
TIANJIN CITY THIRD CENT HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the deficiencies of the prior art, overcome the shortcomings of common polymer materials such as poor biocompatibility, poor anticoagulant activity, and utilize periodate oxidation, diazotization treatment and end-point fixation techniques to provide a multi- Preparation method of polysaccharide molecular fragment composite coating composed of aldehyde-based alginic acid and heparin after diazotization treatment

Method used

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  • Preparation method of polysaccharide molecule fragment composite coating
  • Preparation method of polysaccharide molecule fragment composite coating
  • Preparation method of polysaccharide molecule fragment composite coating

Examples

Experimental program
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Effect test

Embodiment 1

[0029] In the first step, 0.344 g of potassium permanganate was dissolved in 162 ml of deionized water, and 10 ml of 95 wt % concentrated sulfuric acid was slowly added to mix the concentrated sulfuric acid and potassium permanganate evenly.

[0030] In the second step, the polymer material PVC is added to the solution obtained in the first step, and the reaction is fully stirred for 10 minutes.

[0031] In the third step, 2 g of heparin sodium was dissolved in 200 ml of deionized water, 20 mg of sodium nitrite was added, and diazotization was performed at 0° C. for 1.5 hours. Afterwards, the pH of the reaction solution was adjusted to 7.0 to terminate the reaction, and the polysaccharide fragment A was obtained by dialysis through a 7000Da dialysis bag and freeze-drying at -80°C.

[0032] The fourth step is to oxidize the alginic acid according to the molar ratio of sodium periodate to sodium alginate unit of 1:10, so that the end of the alginic acid fragment is exposed to al...

Embodiment 2

[0037] In the first step, 0.328 g of potassium permanganate was dissolved in 144 ml of deionized water, and 20 ml of 95% concentrated sulfuric acid was slowly added to mix the concentrated sulfuric acid and potassium permanganate evenly.

[0038] In the second step, the polymer material is added to the solution obtained in the first step, and the mixture is stirred and fully reacted for 1 min.

[0039] In the third step, 2 g of sodium heparin was dissolved in 200 ml of deionized water, 20 mg of sodium nitrite was added, and diazotization was performed at 0° C. for 2 hours. Afterwards, the pH of the reaction solution was adjusted to 7.0 to terminate the reaction, and the polysaccharide fragment A was obtained by dialysis through a 7000Da dialysis bag and freeze-drying at -80°C.

[0040] The fourth step is to oxidize the alginic acid according to the molar ratio of sodium periodate and sodium alginate at 3:10, so that the end of the alginic acid fragments is exposed to aldehyde gr...

Embodiment 3

[0044] In the first step, 0.328 g of potassium permanganate was dissolved in 144 ml of deionized water, and 20 ml of 95% concentrated sulfuric acid was slowly added to mix the concentrated sulfuric acid and potassium permanganate evenly.

[0045] In the second step, the polymer material is added to the solution obtained in the first step, and stirred and fully reacted for 8 minutes.

[0046] In the third step, 2 g of heparin sodium was dissolved in 200 ml of deionized water, 20 mg of sodium nitrite was added, and diazotization was performed at 0° C. for 1.5 hours. Afterwards, the pH of the reaction solution was adjusted to 7.0 to terminate the reaction, and the polysaccharide fragment A was obtained by dialysis through a 7000Da dialysis bag and freeze-drying at -80°C.

[0047] The fourth step is to oxidize the alginic acid according to the molar ratio of sodium periodate and sodium alginate unit of 1:10, so that the end of the alginic acid fragment is exposed to the aldehyde g...

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Abstract

The invention discloses a preparation method of a polysaccharide molecule fragment composite coating. The preparation method comprises the following steps: soaking a high polymer material in a sulfuric acid solution of potassium permanganate for acidizing; placing the material in a polyethyleneimine solution to obtain an amination-modified surface; dissolving heparin sodium in deionized water, adding nitrous acid and carrying out low-temperature diazotization treatment on the heparin sodium to obtain a polysaccharide fragment A; oxidizing sodium alginate so that the aldehyde group at the tail end of the alginic acid fragment is exposed to obtain polyaldehyde sodium alginate, i.e. a polysaccharide fragment B; and preparing the reaction solution of the polysaccharide fragment A and the polysaccharide fragment B, and placing the material with the amination-modified surface in the reaction solution so as to obtain the composite coating by an endpoint fixing method. In the coating, sodium alginate and heparin group are fixed on the surface of the material, so that the contact area of the sodium alginate and heparin group with blood is enlarged to effectively exert the properties of the sodium alginate and heparin group; and the endpoint fixing method is convenient to implement and the advantages of firm bonding and good space conformation are achieved, and biocompatibility and anticoagulant activity can be effectively improved.

Description

technical field [0001] The invention relates to the technical field of polymer material surface coatings, in particular to a preparation method for modifying polymer material surface coatings by utilizing polysaccharide molecular fragments. Background technique [0002] The application of biomedical materials faces two major problems: biocompatibility and blood compatibility. Coating technology improves the biocompatibility and anticoagulant activity of the surface of biomedical materials by pre-modifying the surface of the material. Heparin is a mucopolysaccharide with a molecular weight of 5000-40000. It is a mixture of negatively charged linear polysaccharides. The most important property of heparin is its anticoagulant properties. The anticoagulant activity of heparin comes from its ability to interact with various Coagulation inhibitors interact to achieve the purpose of anticoagulation by accelerating or increasing the anticoagulant activity of these inhibitors, but t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/10A61L31/16A61L29/08A61L29/16A61L27/34A61L27/54A61L33/10C08J7/04C08B37/10C08B37/04
Inventor 李彤高文卿周秦胡晓旻段大为于美丽李鑫宁萌
Owner TIANJIN CITY THIRD CENT HOSPITAL
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