Method for detecting liuwei caragana preparation
A technology of Caragana liuwei and a detection method, which is applied in the field of detection of traditional Chinese medicine preparations, can solve the problems that there is no detection method for Caragana liuwei preparations, and achieves the effects of strong specificity and good reproducibility.
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experiment example 1
[0064] Experimental example 1: Screening experiment of thin-layer chromatography conditions of Amla emblica
[0065] (1) Instrument
[0066] Electronic scales, Erlenmeyer flasks with stoppers, pipettes, ultrasonic extractors, funnels, filter papers, evaporating dishes, spotters, silica gel G plates, chromatography cylinders, spray bottles, hair dryers.
[0067] (2) Control medicinal materials
[0068] Amla emblica reference medicinal material (batch number: 121289-200301) was purchased from China National Institute for the Control of Pharmaceutical and Biological Products.
[0069] (3) Reagents
[0070] Methanol, ether, acetone, petroleum ether (60-90°C), ethyl acetate, formic acid, ferric chloride.
[0071] (4) Inspection method
[0072] Selection of extraction solvent: use ether, acetone, methanol and other solutions as extraction solvents;
[0073] Selection of extraction methods: Ultrasonic extraction and cold extraction are used respectively;
[0074] The choice of ...
experiment example 2
[0079] Experimental example 2: Screening experiment of Honglian's TLC conditions
[0080] (1) Instrument
[0081] Electronic scales, Erlenmeyer flasks with stoppers, pipettes, ultrasonic extractors, funnels, filter papers, evaporating dishes, spotters, polyamide films, chromatography cylinders.
[0082] (2) Control medicinal materials
[0083] Honglian reference medicinal material (batch number: 121242-200401) was purchased from China Institute for the Control of Pharmaceutical and Biological Products.
[0084] (3) Reagents
[0085] Methanol, ethyl acetate, ether, acetic acid, water.
[0086] (4) Inspection method:
[0087] Selection of extraction solvent: use methanol, ethyl acetate, ether and other solutions as extraction solvents;
[0088] Selection of extraction methods: Ultrasonic extraction and cold extraction are used respectively;
[0089] The choice of developer: use methanol-water (2:7), methanol-acetic acid-water (2:1:7) as developer respectively;
[0090] Th...
Embodiment 1
[0095] [Example 1] Preparation of Caragana lilac preparation
[0096] Grind 150g Tibetan Caragana / Ghostjian Caragana, 100g Pashaga, 130g Emlical, 100g Dwarf Corydalis, 100g Songsheng and other pastes, and 100g Honglian into fine powder, sieve, mix well, and add appropriate amount of water to make pills or take the above six flavors, grind them into fine powder, sieve, mix well, add conventional excipients according to the conventional methods of pharmacy, and make clinically accepted oral preparations: granules, pills, capsules, tablets or powder.
[0097] The usage and dosage of the described Caragana liuwei preparation: the daily dosage is 2g of crude drug, twice a day.
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