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Method for carrying out high-density culture on bacillus subtilis

A technology for high-density cultivation of Bacillus subtilis, applied in the field of microbial fermentation, can solve the problems of less reports

Inactive Publication Date: 2012-07-18
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the research on Bacillus subtilis mainly focuses on the antibacterial effect, the separation, purification and characterization of antibacterial substances, the enzyme production characteristics of Bacillus subtilis and the application effect in soybean meal, etc. There are few reports on the fermentation technology

Method used

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  • Method for carrying out high-density culture on bacillus subtilis
  • Method for carrying out high-density culture on bacillus subtilis
  • Method for carrying out high-density culture on bacillus subtilis

Examples

Experimental program
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Effect test

Embodiment 1

[0025] 1. Determination of Growth Curve of Bacillus subtilis

[0026] After activating Bacillus subtilis, inoculate it into beef extract-peptone liquid medium (beef extract 3g, peptone 10g, sodium chloride 5g, distilled water 1000mL, pH7.0-7.2) at an inoculum size of 5% (v / v), The liquid volume is 100mL / 250mL, cultured at 30°C and 200r / min, and the OD at different culture times is measured by spectrophotometry and plate colony counting method 600 value and the number of colonies, with the culture time as the abscissa, respectively with OD 600 The value and the number of colonies are plotted as a growth curve on the ordinate ( figure 1 ). According to the growth curve, the optimal culture time of Bacillus subtilis was 16h.

[0027]

[0028] 2. Optimization of medium formula for Bacillus subtilis

[0029] The effect of different carbon sources, nitrogen sources and inorganic salts on the biomass of Bacillus subtilis cells was studied with beef extract peptone liquid medi...

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Abstract

The invention belongs to the field of microbial fermentation, and particularly relates to a method for carrying out high-density culture on bacillus subtilis, which aims to improve the yield of the bacillus subtilis. According to the method for carrying out high-density culture on the bacillus subtilis, provided by the invention, the bacillus subtilis is adopted as strains, and is activated to be vaccinated into a fermentation culture medium so as to be cultured. Through optimization, the cell biomass of the bacillus subtilis can reach 4.32*109cfu / mL, which is 31.48 times higher than the cell biomass of the bacillus subtilis before optimization (1.33*108cfu / mL). Through research on culture medium formula and culture conditions, the method lays a foundation for industrial fermentation production and further development and utilization of the bacillus subtilis.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and more specifically relates to a high-density culture of Bacillus subtilis ( Bacillus subtilis )Methods. technical background [0002] As feed additives, antibiotics play a positive role in the prevention of livestock and poultry epidemics, but the widespread use of antibiotics has brought about a series of problems such as environmental pollution, residual toxicity, drug resistance of bacteria, and threats to human and animal safety. In addition, antibiotics will inhibit and kill the beneficial flora in the intestinal tract, resulting in dysbiosis of the normal flora in the intestinal tract, leading to the occurrence of epidemic diseases and double infection, which is harmful to the health of humans and animals. In 1974, Parker proposed a new concept of "probiotics" as opposed to "antibiotics". Probiotics, derived from the Greek word "beneficial to life", refer to the general term of li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/125
Inventor 林娟陈江平叶秀云杨捷李仁宽严芬
Owner FUZHOU UNIV
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