Method for rapid activation of antioxidant activity of 1,000-10,000Da egg white peptide component

A technology of anti-oxidative activity and egg white anti-oxidative peptides, which is applied in the field of intensive processing of agricultural and sideline products and comprehensive utilization of by-products, can solve the problems of unstable activity and unsatisfactory activity of egg white peptide products, and achieve industrialization. Promotion is feasible, pollution is reduced, The effect of less investment in equipment

Inactive Publication Date: 2012-07-18
JILIN UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, the published 5 papers do not affect the creativity and innovation of the patent of the present invention, but it is precisely in these preliminary studies that technical problems such as instability and activity of the egg white peptide product are found, so the patent of the present invention is The research on the activation of egg white antioxidant peptide components by means of high-voltage pulsed electric field technology is the core problem to be solved urgently
[0012] In summary, looking at the relevant research and technical reports at home and abroad, there is no use of high-voltage pulsed electric field technology to activate the antioxidant activity of 1000-10000Da egg white peptide components within 2500μs from the 30% DPPH free radical scavenging rate before treatment. to more than 70% of technological breakthrough research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Use 1000-10000Da egg white antioxidant peptide component dry powder with a DPPH clearance rate of 30% as raw material, fill nitrogen with a purity of more than 98%, and the nitrogen filling rate is 5L / min, according to the 1000-10000Da egg white antioxidant peptide component dry powder The ratio with water is 0.6:100g / mL and mixed evenly; choose an electrode with an electrode length of 0.5mm and an electrode radius of 0.5mm, and then use 50% ethanol and deionized water to clean the material circulation pipeline in the high-voltage pulse electric field twice Finally, after adjusting the electric field intensity of the high-voltage pulse electric field device to 5kV / cm and the frequency to 3000Hz, the constant flow rate of the peptide liquid in the material circulation pipeline of the high-voltage pulse electric field was controlled to be 2.0mL / min, and the calculated The volume of the solution in the pipeline, and calculate the time elapsed for the volume of the sample so...

Embodiment 2

[0031]Use 1000-10000Da egg white antioxidant peptide component dry powder with a DPPH clearance rate of 30% as raw material, fill nitrogen with a purity of more than 98%, and the nitrogen filling rate is 6L / min, according to the 1000-10000Da egg white antioxidant peptide component dry powder The ratio with water is 0.7:100g / mL and mixed evenly; choose an electrode with an electrode length of 1.5mm and an electrode radius of 0.8mm, and then use 60% ethanol and deionized water to clean the material circulation pipeline in the high-voltage pulse electric field twice Finally, after adjusting the electric field intensity of the high-voltage pulse electric field device to 10kV / cm and the frequency to 2700Hz, the constant flow rate of the peptide liquid in the material circulation pipeline of the high-voltage pulse electric field is controlled to be 1.8mL / min, and the calculation value before the high-voltage pulse electric field electrode treatment is The volume of the solution in th...

Embodiment 3

[0033] Use 1000-10000Da egg white antioxidant peptide component dry powder with a DPPH clearance rate of 30% as raw material, fill nitrogen with a purity of more than 98%, and the nitrogen filling rate is 7L / min, according to the 1000-10000Da egg white antioxidant peptide component dry powder The ratio with water is 0.8:100g / mL and mixed evenly; choose an electrode with an electrode length of 3.5mm and an electrode radius of 0.8mm, and then use 70% ethanol and deionized water to clean the material circulation pipeline in the high-voltage pulse electric field twice Finally, after adjusting the electric field intensity of the high-voltage pulse electric field device to 15kV / cm and the frequency to 2400Hz, the constant flow rate of the peptide liquid in the material circulation pipeline of the high-voltage pulse electric field is controlled to be 1.6mL / min, and the calculation value before the high-voltage pulse electric field electrode treatment is The volume of the solution in t...

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Abstract

The invention discloses a method for rapid activation of antioxidant activity of a 1,000-10,000Da egg white peptide component, belongs to the technical field of subsidiary agricultural products deep processing and comprehensive utilization of byproducts thereof, and relates to a method for rapid activation of antioxidant activity of a 1,000-10,000Da egg white peptide component using a high-voltage pulse electric field technique. The method uses a 1,000-10,000Da egg white peptide component with a DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging rate of 30% as a raw material, adopts a high-voltage pulse electric field technique, realizes the key technical invention for peptide activation by electrodes within 2,500mus and rapid increase of the DPPH free radical scavenging rate up to over 70% through preparation of egg white antioxidant peptide liquid, regulation of high-voltage pulse electric field device activation parameters, regulation of peptide liquid constant flow rate, high-voltage pulse electric field activation treatment and vacuum freeze drying processes, and can open a new route for comprehensive utilization of egg white resources.

Description

technical field [0001] The invention belongs to the technical field of intensive processing of agricultural and sideline products and comprehensive utilization of by-products, and relates to a method for rapidly activating the antioxidant activity of 1000-10000Da egg white peptide components by using high-voltage pulsed electric field technology. Background technique [0002] The research and development of antioxidant peptide products has been identified as a hot issue and focus issue by scholars at home and abroad. Peptides derived from natural foods are not only easier to digest and absorb than protein itself, but also promote the body's absorption of other nutrients such as calcium and iron. More importantly, these peptides have the physiological activity of scavenging free radicals. In recent years, domestic and foreign scholars have extracted and isolated peptides with antioxidant activity from soybean, corn, peanut, milk, grass carp and other animal and plant protein...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K2/00C07K1/00
Inventor 林松毅王佳刘静波殷涌光宫新统肇萍
Owner JILIN UNIV
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