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Method for gathering gamma-propalanine in green algae

A technology of aminobutyric acid and green algae, applied in application, food preparation, food science, etc., can solve problems such as difficulty in finding, and achieve the effect of increasing the content

Inactive Publication Date: 2013-08-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the existing green algae GABA enrichment technology is the industrial-grade chemical enrichment technology of adding reaction enzymes and the food-grade microbial enrichment technology using microbial fermentation. Non-chemical and non-microbial enrichment techniques found to be effective in increasing endogenous enzyme activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1, a method for enriching gamma-aminobutyric acid in green algae, the following steps are carried out successively:

[0017] 1) According to the ratio of 20g green algae / 100ml pure water, soak the green algae collected within 24 hours in pure water, then put the green algae and pure water into a polyethylene plastic bag, and vacuum seal; polyethylene The plastic bag is required to withstand the high pressure in the following step 2).

[0018] 2), apply pressure to the inner cavity of the sealed polyethylene plastic bag, so that the green algae in the above-mentioned sealed polyethylene plastic bag are processed under a high pressure of 200MPa for any of the following times: 5, 10, 15min (that is, the holding time 5, 10 or 15 minutes);

[0019] 3) Dry the high-pressure treated green algae obtained in step 2) at room temperature (generally 5-30° C.) in a sterile environment (100,000-grade sterile room) until constant weight.

[0020] The content of γ-aminobut...

Embodiment 2

[0021] Embodiment 2, a method for enriching gamma-aminobutyric acid in green algae, changing the high pressure of 200MPa in step 2) to a high pressure of 300MPa, and the rest are the same as in Example 1. The content of γ-aminobutyric acid in the obtained green algae was determined by high-performance liquid chromatography; details are shown in Table 1-Table 3.

Embodiment 3

[0022] Embodiment 3, a method for enriching gamma-aminobutyric acid in green algae, changing the high pressure of 200MPa in step 2) to a high pressure of 400MPa, and the rest are the same as in Example 1. The content of γ-aminobutyric acid in the obtained green algae was determined by high-performance liquid chromatography; details are shown in Table 1-Table 3.

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Abstract

The invention discloses a method for gathering gamma-propalanine in green algae. The method comprises the following steps: 1, green algae is soaked in purified water and then placed in a sealed container; 2, the green algae in the sealed container is processed for 5 to 15 min under high pressure of 200 to 400 MPa; and 3, the green algae obtained in step 2 and processed under high pressure is aired in a sterile environment until having a constant weight. When the method provided by the invention is adopted, the content of gamma-propalanine in green algae can be improved remarkably.

Description

technical field [0001] The invention relates to the field of functional food development, in particular to a method for enriching gamma-aminobutyric acid in green algae. Background technique [0002] γ-Aminobutyric acid (GABA) is a naturally occurring non-protein amino acid. Animal experiments and human clinical trials at home and abroad in recent years have proved that GABA not only has the effect of inhibiting the excessive excitement of the central nervous system, but also has significant hypotensive effect. Among the food sources that can be used for GABA enrichment, green algae has been recognized as one of the most ideal GABA-enrichment food sources since the end of the last century because of its low processing cost, raw material price, and high-concentration enrichment of GABA. . However, currently there are very few green algae products rich in GABA in the world, mainly because the concentration of GABA enriched by the current green algae GABA enrichment technolog...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/337A23L1/025A23L17/60A23L5/30
Inventor 于勇王春芳朱松明和劲松李建平
Owner ZHEJIANG UNIV
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