Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Ammonia (ammonia ion) content determination method and ammonia (ammonia ion) diagnosis/determination kit

A technology of ammonia ions and reagents, which is applied in the field of medical/food inspection and determination, and can solve problems such as undiscovered blood ammonia determination methods

Inactive Publication Date: 2012-07-11
SUZHOU ANJ BIOTECHNOLOGY CO LTD
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Searching Chinese patents, only found 87105593.7 patent application which discloses a quick-frozen cup for measuring blood ammonia, but did not find an ideal method for measuring blood ammonia

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ammonia (ammonia ion) content determination method and ammonia (ammonia ion) diagnosis/determination kit
  • Ammonia (ammonia ion) content determination method and ammonia (ammonia ion) diagnosis/determination kit
  • Ammonia (ammonia ion) content determination method and ammonia (ammonia ion) diagnosis/determination kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0130] Embodiment 1: the mensuration of ammonia (ammonia ion) content in blood plasma

[0131] 1) Preparation of standard samples

[0132] Dissolve a certain amount of ammonia salt in water or buffer solution, and then adjust the ammonia concentration to 100 micromole / liter;

[0133] 2) Pretreatment of samples to be tested

[0134] Plasma samples are used as samples to be tested without pretreatment;

[0135] 3) Blank sample

[0136] Described water or buffer solution are used as blank sample, and its ammonia concentration is 0 micromole / liter;

[0137] B. Preparation of reagent solution:

[0138] The ammonia diagnosis / measurement reagent of the present embodiment is a single-dose reagent, which contains:

[0139] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0140] Glycerin 1mol / L

[0141] NADPH0.25mmol / L

[0142] Ammonia kinase 16000U / L

[0143] Propionyl-CoA carboxylase 18000U / L

[0144] Phosphogluconate dehydrogenase 12000U / L

[0145] Aden...

Embodiment 2

[0161] Embodiment 2: the mensuration of ammonia (ammonia ion) content in blood plasma

[0162] 1) Preparation of standard samples

[0163] A certain amount of ammonia salt is dissolved in water, and then the concentration of ammonia (ammonia ion) is adjusted to 100 micromole / liter, as a standard sample;

[0164] 2) Preparation of samples to be tested

[0165] Plasma samples are used as samples to be tested without pretreatment;

[0166] 3) Blank sample

[0167] Described water is used as blank sample, and its ammonia (ammonia ion) concentration is 0 micromole / liter;

[0168] B. Preparation of reagent solution:

[0169] Ammonia (ammonia ion) diagnostic / determination reagent is a two-dose reagent, which contains:

[0170] Reagent 1 with the following composition:

[0171] Phosphate buffer 100mmol / L

[0172] NADH0.25mmol / L

[0173] Adenosine triphosphate 5mmol / L

[0174] Methylmalicyl CoA 5mmol / L

[0175] Ribose-5-phosphate 5mmol / L

[0176] Monovalent phosphate 5mmol / L...

Embodiment 3

[0199] Embodiment 3: the mensuration of ammonia (ammonia ion) content in plasma sample

[0200] The mode of operation of this embodiment is the same as embodiment 2, but this embodiment:

[0201] B. Preparation of reagent solution:

[0202] Ammonia (ammonia ion) diagnostic / assay reagent is a three-dose reagent that contains:

[0203]Reagent 1 with the following composition:

[0204] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0205] NADH0.25mmol / L

[0206] Adenosine triphosphate 5mmol / L

[0207] Methylmalicyl CoA 5mmol / L

[0208] Ribose-5-phosphate 5mmol / L

[0209] Monovalent phosphate 5mmol / L;

[0210] Reagent 2 with the following composition:

[0211] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0212] Sodium chloride 2mol / L

[0213] Propionyl-CoA carboxylase 28000U / L

[0214] Phosphogluconate dehydrogenase 50000U / L;

[0215] Reagent 3 with the following composition:

[0216] Tris(carboxymethyl)aminomethane-hydroc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an ammonia (ammonia ion) content determination method, and composition and ingredients of reagents adopted by the ammonia (ammonia ion) content determination method. The ammonia (ammonia ion) content determination method adopts an enzymatic colorimetry and an enzyme-linked immunosorbent assay technology. A technical principle of the ammonia (ammonia ion) content determination method comprises that determination is realized by a series of catalytic reactions of ammonia kinase, alpha-propionyl-CoA carboxylase and phosphogluconate dehydrogenase. The invention also relates to an ammonia (ammonia ion) diagnosis / determination kit. The ammonia (ammonia ion) content determination method provided by the invention has high sensitivity and a small error. The ammonia (ammonia ion) content determination method and the ammonia (ammonia ion) diagnosis / determination kit can be widely utilized for clinical medicine and food examination.

Description

technical field [0001] The present invention relates to the technical field of medical / food inspection and determination, more specifically, the present invention relates to ammonia (ammonia ion) diagnosis / measurement method and kit thereof. Background technique [0002] Ammonia determination methods include micro-diffusion method, ion exchange method, enzymatic method and ammonia electrode method. The most widely used methods are enzymatic method and blood ammonia analyzer analysis method based on ion selective electrode. [0003] The diffusion method is that after the sample is alkalized, NH is released 3 , Titrate the released ammonia with acid, or use the Nessler reaction to form brown-yellow mercuric ammonium iodide for colorimetry. These methods need to be alkalized, and the endogenous ammonia formation will affect the accuracy and precision, so they are rarely used at present; the ion exchange method is more accurate than the diffusion method, and the CV is 8% to 13...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/52G01N21/31G01N21/33C12Q1/48C12Q1/25C12Q1/32
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com