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Method for monitoring preparation of codfish immunoactive peptide on line

An immunoactive peptide, cod technology

Inactive Publication Date: 2012-07-11
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to literature research and novelty searches at home and abroad, there are no reports of biosensors in the enzymatic preparation of bioactive peptides.

Method used

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  • Method for monitoring preparation of codfish immunoactive peptide on line
  • Method for monitoring preparation of codfish immunoactive peptide on line
  • Method for monitoring preparation of codfish immunoactive peptide on line

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0021] 1) Preparation of network training samples. The training sample refers to the use of trypsin to hydrolyze cod protein, and the hydrolysis reaction is carried out by changing the initial substrate concentration and initial protease concentration, and the hydrolysis degree, free glutamic acid and free lysine of each enzymatic hydrolysis reaction are measured respectively. The degree of hydrolysis of the enzymatic hydrolysis reaction was determined by the ninhydrin method; free glutamic acid and free lysine were measured by biosensors.

[0022] 2) The parameters of the artificial neural network are determined. It mainly includes: the number of hidden layers of the network model, the transfer function, the training function, the learning function, the input layer nodes, the output layer nodes, and the number of hidden layer nodes.

[0023] Determination of the number of hidden layers. A three-layer BP network can complete any N-dimensional to M-dimensional mapping, that ...

Embodiment 1

[0035] Example 1: GLU-BP-ANNs model online monitoring and preparation of immune peptides

[0036] Step 1: Establish and train the GLU-BP-ANNs network model.

[0037] 1) Preparation of network training samples. The training sample refers to the use of trypsin to hydrolyze the cod protein, and the hydrolysis reaction is carried out by changing the initial substrate concentration and the initial protease concentration, and the hydrolysis degree and free glutamic acid of each enzymatic hydrolysis reaction are measured respectively. The degree of hydrolysis of the enzymatic hydrolysis reaction was determined by the ninhydrin method; the free glutamic acid was determined by a biosensor.

[0038] 2) The parameters of the artificial neural network are determined. The hidden layer is 1 layer, the logsig transfer function is used between the input layer and the hidden layer, the linear purelin function is used between the hidden layer and the output layer, the network training functio...

Embodiment 2

[0047] Step 1: Establish and train the LYS-BP-ANNs network model.

[0048] 1) Preparation of network training samples. The training sample refers to the use of trypsin to hydrolyze the cod protein, and the hydrolysis reaction is carried out by changing the initial substrate concentration and the initial protease concentration, and the hydrolysis degree and free lysine of each enzymatic hydrolysis reaction are measured respectively. The hydrolysis degree of the enzymatic hydrolysis reaction was determined by the ninhydrin method; the free lysine was determined by a biosensor.

[0049] 2) The parameters of the artificial neural network are determined. One hidden layer, the logsig transfer function is used between the input layer and the hidden layer, the linear purelin function is used between the hidden layer and the output layer, the network training function uses trainlm, the adaptive learning function uses LEARNGDM, and the input layer nodes are respectively the initial enz...

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Abstract

The invention relates to codfish immunoactive peptide and a method for monitoring preparation of the codfish immunoactive peptide on line. A preparation method for the codfish immunoactive peptide comprises the following steps: hydrolyzing codfish protein by using trypsin; and stopping hydrolysis when the hydrolysis degree is 16 to 17 percent. GLU-BP-ANNs and LYS-BP-ANNs network modes are established, so that the method for monitoring preparation of the codfish immunoactive peptide on line is provided. The optimum hydrolysis degree of the codfish protein is determined, so that basis is laid for achieving the codfish immunoactive peptide with the optimum immune effect. After the optimum hydrolysis degree is determined, the on-line monitoring method is established, so the hydrolysis reaction degree can be monitored on line through a biosensor-artificial neural network model. Even if the hydrolysis conditions are fluctuated, for example temperature, pH value, stirring speed and the like are changed, the hydrolysis reaction degree can still be monitored accurately. Therefore, the method has a good popularization and application prospect.

Description

technical field [0001] The invention belongs to the technical field of biotechnology enzyme engineering, and specifically relates to an online monitoring method for the preparation of cod immunoactive peptides, which can dynamically monitor the hydrolysis process during the preparation of bioactive peptides, realize the visualization of hydrolysis, and obtain the target activity to the greatest extent peptide. Background technique [0002] In recent years, it has been found that some natural immune active peptides such as thymosin can induce lymphocyte differentiation, proliferation and maturation, and improve the immune activity of immunocompromised mice. However, the content of natural immune active peptides in organisms is small, and the preparation cost is high and the price is expensive. Although chemical methods can synthesize a large number of immunoactive peptides, the chemically synthesized immunoactive peptides have many side reactions and by-products, and their a...

Claims

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Application Information

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IPC IPC(8): C12P21/06G01N27/327G06N3/02
Inventor 侯虎李八方赵雪张朝辉
Owner OCEAN UNIV OF CHINA
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