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Method for extracting cordycepin and cordyceps polysaccharide from cordyceps militaris

An extraction tank and column extraction technology, which is applied in the field of extracting cordycepin and cordyceps polysaccharides from Cordyceps militaris, can solve the problems of waste of resources and low purity, and achieve the effect of avoiding decomposition

Inactive Publication Date: 2012-07-11
LIAONING HUAXIA SANBAO BIO RESOURCE TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The task of the present invention is to provide a method for extracting cordycepin and polysaccharide from Cordyceps militaris which solves the problems of resource waste and low purity by adjusting the amount of water added and temperature. method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] First carry out water extraction: grind the Cordyceps militaris culture medium into powder and put it into the extraction tank, then add water whose quality is 18 times the quality of the Cordyceps culture medium powder in the extraction tank, and heat the extraction tank so that the When the water temperature reaches 58°C, the aqueous solution is extracted by heat reflux, and then the extracted aqueous solution is filtered and concentrated by decompression;

[0012] Alcohol extraction: put the extract concentrated under reduced pressure into a container, add edible alcohol 6 times that of the concentrated extract into the container, and then use a centrifuge to separate the supernatant and sediment;

[0013] Column extraction supernatant: first concentrate the supernatant separated by the centrifuge, because the alcohol in the supernatant is small in quality and can float on the upper part, recover the upper part of the alcohol and then add the concentrated soluti...

Embodiment 2

[0017] The method for extracting cordycepin and cordyceps polysaccharide in this embodiment is basically the same as that in Example 1, the difference is that in the water extraction step, water with a quality 12 times that of the Cordyceps culture medium powder is added to the extraction tank, and when the extraction tank is heated Make the water temperature reach 55°C, add 10 times of edible alcohol to the container during alcohol extraction, add 8 times of water to the container when column extracting the precipitate, and then add 2.5 times of edible alcohol for alcohol precipitation.

Embodiment 3

[0019] The method for extracting cordycepin and cordyceps polysaccharide in this embodiment is basically the same as that in Example 1, the difference is that in the water extraction step, water with a quality 15 times that of the Cordyceps culture medium powder is added to the extraction tank, and when the extraction tank is heated Make the water temperature reach 57°C, add 10 times of edible alcohol to the container during alcohol extraction, add 10 times of water to the container when column extracting sediment, and then add 3 times of edible alcohol for alcohol precipitation.

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Abstract

The invention relates to a method for extracting cordycepin and cordyceps polysaccharide from cordyceps militaris. The method comprises the following steps of: water extraction, alcohol extraction, column extraction of supernate and column extraction of sediment. In the water extraction step, the water temperature is set between 55-58DEG C, namely active ingredients in cordyceps can be fully dissolved, and the cordycepin can be prevented from being decomposed. Therefore, resource waste is avoided when the cordycepin and the cordyceps polysaccharide are extracted at the water temperature.

Description

technical field [0001] The invention relates to a method for extracting cordycepin and cordyceps polysaccharide from Cordyceps militaris. Background technique [0002] Cordycepin is the main active ingredient in Cordyceps militaris, which has various pharmacological effects such as antitumor, antibacterial and antiviral, immune regulation, and scavenging free radicals. Cordyceps polysaccharide is a polysaccharide composed of mannose, cordycepin, adenosine, galactose, arabinose, xylose, glucose, and fucose. Cordyceps polysaccharide also has the effect of improving human immune function and increasing white blood cells. It is used to treat various diseases such as malignant tumors and tuberculosis. The effect of blood lipids. Since the artificially cultivated Cordyceps militaris, Cordyceps militaris, contains relatively large amounts of polysaccharides and cordycepins, the extraction of cordycepin and polysaccharides from Cordyceps militaris has been recognized by the publi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H19/16C07H1/08C08B37/00
Inventor 李福全刘明辉李洪霞孙红霞
Owner LIAONING HUAXIA SANBAO BIO RESOURCE TECH DEV CO LTD
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